Streptomycin – Like Antibiotic from Streptomyces spp. Isolated from Mount Everest Base Camp

(Published in Nepal Journal of Science and Technology 9(2008) 73-75)

Streptomycin – Like Antibiotic from Streptomyces spp. Isolated from Mount Everest Base Camp

Jyotish Yadav1, Upendra Thapa Shrestha2, Kiran Babu Tiwari1,2, Gyan Sundar Sahukhal2 and Vishwanath Prasad Agrawal2

1Universal Science College, Pokhara University, Maitidevi, Kathmandu
2Research Laboratory for Biotechnology and Biochemistry (RLABB), Maitidevi, Kathmandu

Abstract

Streptomyces spp. (Lob18.2b), isolated from soil sample from Everest Base Camp, was obtained from Research Laboratory for Biotechnology and Biochemistry (RLABB). The isolate was found to inhibit Salmonella paratyphi, Salmonella typhi, Proteus mirabilis, Proteus vulgaris, Shigella sonnei, Klebsiella pneumoniae, Klebsiella oxytoca, Escherichia coli, Bacillus subtilis and Staphylococcus aureus on primary screening. Secondary screening was done using fermented starch casein broth of the streptomycete to its stationary phase culture. The antibacterial was highly effective against all susceptible Gram negative bacteria except Proteus spp. Gram positive bacteria were relatively lesser sensitive. Pseudomonas aeruginosa was resistant to the agent. Antibacterial activity of aqueous fraction obtained from fermented broth of the streptomycete culture was more effective than that of organic fraction same extract. Thin layer chromatography revealed that the test compound was relatively nonpolar compared to the known antibiotics. Among the tested standard antibiotics, the chemical characteristic of the antibacterial agent was comparable to streptomycin.

Keywords: aminoglycoside, antibacterial agent, fermentation, secondary screening, thin layer chromatography