MICROBIAL ANALYSIS (COLIFORM AND VIBRIO CHOLERAE) OF DRINKING WATER COLLECTED FROM BHAKTAPUR MUNICIPALITY WARD NO.-10

(Submitted for Publication)

Hishila Sujakhu¹ and Upendra Thapa Shrestha¹

1. Khwopa College, Tribhuvan University, Dekocha, Bhaktapur.

ABSTRACT

Forty water samples were collected from Bhaktapur Municipality ward no. 10, one of the most water borne suffering regions, following standard methods described by WHO. All samples were transferred to Microbiology laboratory of Khwopa College, Dekocha, Bhaktapur and proceeded immediately for Microbial analysis. The coliform density in the water samples were determined by Most Probable method (MPN) followed by microscopy, colonial morphology and biochemical characterization. Subsequently, Vibrio cholerae, a causative agent of Cholera was detected in the same samples by using Thiosulphate citrate bile-salt sucrose (TCBS) agar, a selective media for Vibrio sps., followed by oxidase test. Among forty water samples, 87.5 % (35 water samples) contained coliforms and half of which contained feacal coliforms, E coli. Remaining 12.5 % (5 water samples) contained no coliforms. All water samples except two samples were free of Vibrio cholerae.

Key words: MPN, Coliforms, TCBS agar, Vibrio cholerae and Oxidase test.

A project work submitted to Department of Environment Science, Khwopa College, Dekocha, Bhaktapur as a course of M. Sc. First year.