A Complete Note on Instrumentation (Analytical Biochemistry)
UPENDRA THAPA SHRESTHA
A Complete Note
on Instrumentation (Analytical Biochemistry)
First Edition,
November-2016
© Author (Shrestha U.T.)
UPENDRA THAPA SHRESTHA, M.Sc.
Microbiology (TU)
Head of Microbiology
Department: Kantipur College of Medical Science, Sitapaila, KTM
(B.Sc. and M.Sc. Microbiology affiliated to TU;
CMLT and D-Pharmacy affiliated to CTEVT)
Deputy Director: Research Laboratory for Biotechnology and
Biochemistry (RLABB)
(Research Scientist)
Faculty: Khwopa College (B.Sc. and M.Sc. Environment
Science), Dekocha, Bhaktapur
PGT: Sainik Awasiya Mahavidhyalaya, Sallaghari, Bhaktapur
Email: upendrats@gmail.com
Publisher:
All
rights reserved.
No part of this book may be reproduced
in any form, by photocopy, microfilm, scanning or any other means, or
incorporated into any information retrieval system, electronic or mechanical,
without the written permission of the author.
Thank you!
UNIT-I
·
1.1 Physiological
Solutions
·
1.2
Cell Disruption
o
Mechanical
Cell Disruption
o
Non-mechanical
Cell Disruption
§ Physical Methods
§ Chemical Methods
§ Biological Methods
UNIT-II
·
Protein
Purification
o
Background
knowledge about protein
o
Protein
Isolation/Precipitation
o
Protein
Purification
o
Protein
Determination
o
Protein
Assay
UNIT-III
·
Centrifugation
o
Principle
of Centrifugation
o
Centrifuges
and its types
o
Differential
Centrifugation
o
Density
Gradient Centrifugation
UNIT-IV
·
Chromatography
o
Paper
Chromatography
o
Thin
Layer Chromatography
o
Column
Chromatography
§ Size Exclusion Chromatography
§ Ion Exchange Chromatography
§ Affinity Chromatography
§ Gas Chromatography
§ High Performance Liquid Chromatography (HPLC)
UNIT-V
·
Electrophoresis
o
Principle
of Electrophoresis
o
Media
used in Electrophoresis
o
Agarose
Gel Electrophoresis
o
Isoelectric
Focus Gel Electrophoresis
o
SDS-PAGE
o
2D
Gel Electrophoresis
o
Western
Blotting
UNIT-VI
·
Spectroscopy
o
UV-Visible
Spectrophotometry
§ Colorimeter/Photometer/Spectrophotometer
o
Spectroflouremetry
o
Molecular
Luminescence Spectroscopy
o
Atomic
Spectroscopy
§ Atomic Absorption Spectroscopy
§ Atomic Emission Spectroscopy
§ Atomic Florescent Spectroscopy
o
Mass
Spectroscopy
o
Nuclear
Magnetic Resonance
UNIT-VII
·
Microscopy
o
Electron
Microscopy
§ Transmission Electron Microscopy
§ Scanning Electron Microscopy
o
Phase
Contrast Microscopy
o
Fluorescent
Microscopy
o
Gel-Doc
System
UNIT-VIII
·
Polymerase
Chain Reaction
·
Microarrays
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