Upendra Thapa
Shrestha # 1 2, Sabnum Shrestha # 1, Nabaraj Adhikari 1 2, Komal Raj Rijal 2, Basudha Shrestha 3, Bipin Adhikari 4, Megha Raj
Banjara 2, Prakash Ghimire 2
Affiliations expand
·
PMID: 32606839
PMCID: PMC7320882
·
DOI: 10.2147/IDR.S250591
Free
PMC article
Abstract
Introduction: Extended-spectrum β-lactamases (ESBL) among Gram-negative
bacteria, predominantly Escherichia coli (E. coli), in
Nepal, have been rising. The main objectives of this study were to determine
the prevalence of uropathogenic E. coli, antibiotic resistance,
ESBLs, ABLs (AmpC type β-lactamases), MBLs (metallo-β-lactamases) and KPCs (Klebsiella
pneumoniae carbapenemases) and their correlation with plasmid
profiling patterns among patients with urinary tract infections in a tertiary
hospital in Kathmandu, Nepal.
Methods: The mid-stream urine samples collected from patients were
inoculated in cystine-lactose-electrolyte-deficient (CLED) agar plates. E.
coli producing ESBLs, ABLs, MBLs/KPC were identified phenotypically
using standard microbiological methods. Plasmids were extracted by alkaline
lysis method from E. coli isolates and profiled using agarose
gel electrophoresis.
Results: Out of the total 2661 urine samples, E. coli were
isolated in 64.34% (507/788), among which 170 (33.53%) were multidrug-resistant
(MDR) isolates. All MDR isolates were resistant to amoxicillin and
third-generation cephalosporins but were highly sensitive to imipenem (94.12%,
160/170), amikacin (92.94%, 158/170) and nitrofurantoin (86.47%, 147/170).
Among 170 MDR isolates, 78.2% (133/170) were ESBLs, 46.3% (50/170) were AmpC,
11.2% (19/170) were MBL and 0.6% (1/170) were KPC producers. Coproduction of
β-lactamases was detected in 24.12% (41/170) of isolates. E. coli isolates
showed one plasmid (>33.5 kb), which was present in all the isolates.
Overall, 44 different plasmid profile groups were identified based on molecular
weight and number of plasmids. β-Lactamase producers were relatively resistant
to the higher number of antibiotics tested (≤10) than non-producers (≤8), and
the number of plasmids were higher in β-lactamase producers (≤7) than those in
non-producers (≤5).
Conclusion: The higher prevalence of the ESBLs, AmpCs, KPCs and MBLs along
with their coproduction in E. coli isolates highlights the
importance of routine surveillance of ESBLs, AmpCs, KPCs and MBLs in microbiology
laboratories using various phenotypic methods.
Keywords: AmpC type β-lactamases; ESBL; KPC; Klebsiella pneumoniae
carbapenemases; MBL; antibiotic resistance; extended-spectrum β-lactamases;
metallo-β-lactamases; uropathogenic Escherichia coli.
Fulltext Article: Download
28 comments:
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before buying anything online, try get your tools ready.. you can check on him on telegram weather he is still in business
Telegram- @darknetangel
icq chat- 746825067
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SPARK! CRYPTOCURRENCIES PRIVATE KEYS SOFTWARE - $500 Bitcoin
SPARK! CREDIT CARD HACK SOFTWARE - $500 Bitcoin
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www.vidlii.com/watch?v=2nJ44BSyS3P
www.vidlii.com/watch?v=lUu54scCYU9
blackeels@outlook.com
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Telegram= @leadsupplier @killhacks @jacobfullz
ICQ= 752822040 / @killhacks
Email= hacksp007 @ dnmx.org
FRESH FULLZ/PROS AVAILABLE
CC with CVV
SSN DOB DL
High Credit Scores Pros
Business EIN Fullz
Dumps with PIN Codes Track 101 & 202
DL Scan Front & Back
Fullz for KYC, PUA, UI, Tax Refund
USA, UK, CANADA Fullz Available
Fresh & Legit stuff
No refund only replacement
This is for anyone seeking to recover their data or funds lost to scammers on the internet. I sent out $132,000.00 worth of BTC to this broker named E29 Crypto Marketers. My experience was a good business proposal turned sour. l was added to a crypto learning and trading group on Telegram. I thought it was real, unfortunately, they are like other fraudsters found on the internet and online romance apps, I only knew about their fraudulent activities after losing my investment to E-29 marketers. l would have lost all my invested funds to this online scam scheme. with the quick help of these professionals in reclaiming stolen money. I found their reviews on Google, After my research about them, I concluded they were the perfect recovery expert to hire to recover my stolen cryptos. I never regret coming in contact with CYBER GENIE HACK PRO team. Within the short period I spent conversing with them during the recovery process, I learned a lot more than the four walls of a school. They can be reached via their details below: Watsapp; +1 (252) 512-0391 Telegram; CYBERGENIEHACKPRO... Web; cybergeniehackpro (.) xyz/
FRESH&VALID SPAMMED USA DATABASE/FULLZ/LEADS
SSN PROS
****Contact****
*ICQ :748957107
*Telegram : @James307
<><><><><><><>
USA SSN FULLZ WITH ALL PERSONAL DATA+DL NUMBER
-FULLZ FOR PUA-SBA-UBER-DOORDASH
-FULLZ FOR TAX REFUND
$2 for each fullz/lead with DL num discount for bulk order
$1 for each SSN+DOB--discount for bulk order
$5 for each with Premium info--(income detail,employment detail,Good credit score)
ID's Photos For any state (back,front,selfie & ssn )
Young age data
Any age range data available
UK data-Canada data
(Price can be negotiable if order in bulk)
<><><><><><><><><><><>
+High quality and connectivity
+If you have any trust issue before any deal you may get few to test
+Every leads are well checked and available 24 hours
+Fully cooperate with clients
+Any invalid info found will be replaced
+Payment Method(BTC,USDT,ETH,LTC & PAYPAL)
+Fullz available according to demand too i.e (format,specific state,specific zip code & specifc name etc..)
<><><><><><><><><><>
+US cc Fullz
+(Dead Fullz)
+(Email leads with Password)
+(Dumps track 1 & 2 with pin and without pin)
+Hacking & Carding Tutorials
+Smtp Linux
+Safe Sock
+Server I.P's
+HQ Emails with passwords
<><><><><><><><>
*Let's do a long term business with good profit
*Contact for more details & deal
****Contact****
*ICQ :748957107
*Telegram :@James307
FRESH FULLZ LEADS UPDATED-2024 FRESH SPAMMED
SSN DOB DL ADDRESS USA
SIN DOB DL ADDRESS MMN CANADA
NIN DOB DL ADDRESS SORT CODE UK
T3l3gram- @ killhacks | @ leadsupplier
Wh@ts App - +1.. 727.. 788.. 6129
I C Q - 752822040 | @ killhacks
E mail - bigbull0334 (at) onion mail . org
Skyp3 - @ peeterhacks
FULLZ AVAILABLE IN BULK QUANTITY
Updated fresh spammed Leads
Fully verified & genuine info
All USA UK Canada States available
Samples will be provided too if you want in bulk
USA STUFF LISTED BELOW:
===============================
SSN DOB ADDRESS FUllz Leads
SSN DOB DL ADDRESS EMPLOYEE & BANK INFO LEADS Fullz
SSN DOB ADDRESS MMN EMAIL & PHONE INFO FULLZ
SSN DOB DL ADDRESS Fullz with Issue & Expiry Dates
High Credit Scores Fullz Pros
Real DL|ID Scan Front Back with Selfie & SSN
Business EIN Company Info Fullz
Young & Old Age Fullz (2002 above & 1960 below)
Business Owner Fullz with SSN
EIN Info with DL Front Back Selfie & SSN
CC Fullz with CVV & Billing Address with SSN
Dumps With Pins Track 101 & 202
Fullz for KYC & UberEats or Doordash Accounts
Tax Return Filling Fullz with W-2 Form
Business Email Leads
SBA|PUA|UI|Loan Filling Fullz
Passport Photos With Selfie
Cars Database with Docs
Email|Phone Numbers|Employement|Banking Leads
EIN Look-Up|SSN Look up Service
You can get specific info of any USA State
Like specific Age|Gender|City|Zip Codes|Bank Name
Loan Methods|Cash out Methods Available as well
#SSN #SSNDOBDL #SellSSN #CCShop #CCSELLCVV #ShopSSNDOBDLADDRESS #FULLZ #SSNFULLZ
#REALDLSCAN #YoungAgeFullz #Fullzseller #USAFULLZ #FULLZUSA #SellerSSNDOB #ShopSSNDOB
#BusinessFullzUSA #USAPros #USALeads #EINFullz #taxreturn #W2form #HighCSPRos #HighCreditFullz
#ReturnFilling #UberEatsFullz #DoordashFullz #KYCStuff #SSNLookup #einlookup #ssnpros #shopssn-dl
#ssnbankemployee #ssnemployee #ssnfullzbank
CONTCT US FOR MORE DETAILS & DEALS
T3l3gram- @ killhacks | @ leadsupplier
Wh@ts App - +1.. 727.. 788.. 6129
I C Q - 752822040 | @ killhacks
E mail - bigbull0334 (at) onion mail . org
Skyp3 - @ peeterhacks
CANADA STUFF LISTED BELOW:
=====================================
SIN DOB ADDRESS Fullz Leads
SIN DOB DL ADDRESS MMN INFO LEADS Fullz
High Credit Scores Fullz Pros
Real DL|ID Scan Front Back with Selfie
CC Fullz with CVV & Billing Address
Dumps With Pins Track 101 & 202
Business Email Leads
Passport Photos With Selfie
All Canada States Info available
#SIN #SINDOBDL #SellSIN #SINMMNFULLZ #MMNPROSSIN #MMNSIN #CCShop #CCSELLCVV #ShopSINDOBDLADDRESS #FULLZ #SINFULLZ
#REALDLSCAN #YoungAgeFullz #Fullzseller #CANADAFULLZ #FULLZCANADA #SellerSINDOB #ShopSINDOB
#BusinessFullzCanada #CanadaPros #CanadaLeads#HighCSPRos #HighCreditFullz
#KYCStuff #sinpros #shopsin-dl
UK (United Kingdom) STUFF LISTED BELOW
NIN DOB ADDRESS FUllz Leads
NIN DOB DL ADDRESS SORT CODE INFO LEADS Fullz
High Credit Scores Fullz Pros
Real DL|ID Scan Front Back with Selfie
CC Fullz with CVV & Billing Address
Dumps With Pins Track 101 & 202
Business Email Leads
Passport Photos With Selfie
All UK States Info available
#NIN #NINDOBDL #SellNIN #CCShop #CCSELLCVV #ShopNINDOBDLADDRESS #FULLZ #NINFULLZ
#REALDLSCAN #YoungAgeFullz #Fullzseller #UKFULLZ #FULLZUK #SellerNINDOB #ShopNINDOB
#BusinessFullzUK #UKPros #UKLeads#HighCSPRos #HighCreditFullz #UKFULLZSORTCODE #shopukpros
All stuff will be provided fresh & never sold
Fast Delivery after payment proof
Payment Upfront in crypto only
No refund only Replacement
Contact Below:
T3l3gram- @ killhacks | @ leadsupplier
Wh@ts App - +1.. 727.. 788.. 6129
I C Q - 752822040 | @ killhacks
E mail - bigbull0334 (at) onion mail . org
Skyp3 - @ peeterhacks
SELL FRESH SSN SIN NIN WITH DOB DL ADDRESS
USA UK CANADA ALL STATES
SPAMMED DATABASE NOT GENERATED
GUARANTEE WITH FAST DELIVERY
NIN DOB ADDRESS FUllz Leads
NIN DOB DL ADDRESS SORT CODE INFO LEADS Fullz
SSN DOB ADDRESS FUllz Leads
SSN DOB DL ADDRESS EMPLOYEE & BANK INFO LEADS Fullz
SSN DOB ADDRESS MMN EMAIL & PHONE INFO FULLZ
SIN DOB ADDRESS Fullz Leads
SIN DOB DL ADDRESS MMN INFO LEADS Fullz
High Credit Scores Fullz Pros
Young & Old Age Fullz (2002 above & 1960 below)
Business Owner Fullz with SSN
Passport Photos With Selfie
Cars Database with Docs
Email|Phone Numbers|Employement|Banking Leads
EIN Look-Up|SSN Look up Service
All stuff will be provided fresh & never sold
Fast Delivery after payment proof
Payment Upfront in crypto only
No refund only Replacement
FOR MORE DETAILS
T3l3gram- @ killhacks | @ leadsupplier
Wh@ts App - +1.. 727.. 788.. 6129
E mail - bigbull0334 @ onion mail . org
Skyp3 - @ peeterhacks
#SSN #SSNDOBDL #SellSSN #CCShop #CCSELLCVV
#SIN #SINDOBDL #SellSIN #SINMMNFULLZ #MMNPROSSIN #MMNSIN
#NIN #NINDOBDL #SellNIN #CCShop #CCSELLCVV #ShopNINDOBDLADDRESS
FULLZ AVAILABLE FRESH SSN DOB DL ADDRESS 2024 UPDATED
USA UK CANADA All States info available
Fresh & Legit info
Verified & Genuine Leads
Spa-mmed stuff not generated
Tele Gram - @ leadsupplier - @ killhacks
Whats App - +1.. 727.. 788.. 6129..
Skype - @ peeterhacks
E mail - cyber.zoneuniverse at g mail . com
USA SSN DOB DL ADDRESS
UK NIN DOB DL ADDRESS SORT CODE
CANADA SIN DOB DL ADDRESS MMN PHONE EMAIL
High Credit Scores Fullz Pros 700+
Real DL|ID Scan Front Back with Selfie & SSN
Young & Old age info
CC with CVV & Billing Address
Passport Photos with Selfie
Business EIN Company Pros
EIN Lookup
Fullz for KYC|UberEats|Doordash
W-2 Form with DL front back & Docs
SBA|PUA|UI|Credit Card|Loan Applying Fullz Info
Dumps with Pin Track 101 & 202
#SSN #SSNDOBDL #SellSSN #CCShop #CCSELLCVV #BusinessFullzUSA
#SIN #SINDOBDL #SellSIN #SINMMNFULLZ #Fullzseller #CANADAFULLZ
#NIN #NINDOBDL #SellNIN #UKFULLZ #FULLZUK #SellerNINDOB
Specific Info you can also get
Like Age|City|State|Zip|Gender
Updated Database 2024, not sold before
Contact Details are mentioned above
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