Antibiogram and Biofilm Development among Klebsiella pneumoniae from Clinical Isolates
Subash Paudel1, Laxmi Parajuli1, Prashanna
Adhikari1, Sanjay Singh K.C.2, Upendra Thapa Shrestha3, Pradeep Kumar Shah1*
1Department
of Microbiology, Tri-Chandra Multiple Campus, Kathmandu, Nepal
2Department
of Microbiology, SahidGangalal National Heart Center, Kathmandu, Nepal
3Central
Department of Microbiology, Kathmandu, Nepal
*Corresponding author: Pradeep Kumar
Shah; Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan
University, Kathmandu, Nepal. Email: pkshah210@gmail.com
ABSTRACT
Objectives: This study was aimed to evaluate antibiotic
resistance pattern and biofilm formation in K. pneumoniae strains
isolated from different clinical specimens and to study on association of drug resistance
pattern with biofilm formation.
Methods: A total of 944 clinical samples from patients attending Sahid
Gangalal National Heart Center were processed from September 2019 to March 2020
to identify possible bacterial pathogens following standard microbiological
procedures. K. pneumonaie isolates were further subjected to antibiotic
susceptibility testing using modified Kirby Bauer disc diffusion technique.
Biofilm formation was evaluated by tissue culture plate technique.
Results: Of the total 944 samples, 146 (15.47%) samples showed bacterial
growth, among which 35 (23.97%) were K. pneumoniae. Out of 35 K.
pneumoniae isolates, 16 (45.71%) were multidrug-resistant and 15 (42.86%)
were extensively drug-resistant. Twenty-one (60%) K. pneumoniae feebly
produced biofilm. Significant association was observed between biofilm
production and exhibition of multidrug resistance (p < 0.05).
Conclusion: Prevalence of antibiotics resistant K.
pneumoniae in hospital setting is high and alarming. Significant association
between drug resistance pattern and biofilm production implicates need of an
immediate response to limit growth and spread of drug resistant microbes in
clinical settings.
Keywords: Kleibsella pneumoniae, multidrug resistance, biofilm,
antibiotic susceptibility test, Nepal
Citation: Paudel et al. Antibiogram
and Biofilm Development among Klebsiella pneumoniae from Clinical
Isolates. 2021, TUJM 8(1): 83-92. DOI: https://doi.org/10.3126/tujm.v8i1.41198
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