# ECTMIH2023, Utrecht, Netherlands #Oral presentation

 OCCURRENCE OF β-LACTAM RESISTANT BACTERIAL GENES FROM CLINICAL, ENVIRONMENTAL, AND POULTRY ISOLATES

92

Upendra Thapa Shrestha¹, Dev Joshi¹, Manash Shrestha², Nabaraj Shrestha³, Komal Rijal¹, Prakash Ghimire¹

¹Tribhuvan University, Institute of Science and Technology

²APMEN
³Central Veterinary Laboratory (CVL)

 

Introduction

The widespread and irrational use of antibiotics in empirical therapy and veterinary practices are of great concern in the developing world. Besides, the improper management of antimicrobial waste adds to the emergence of drug-resistant pathogens. Although there is regular reporting of the increased resistance among clinical isolates, the occurrence of the resistance genes among bacteria from the environment and animal origins is not well documented in our contest. 

Methods

Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were isolated from different samples and were subjected to antimicrobial susceptibility testing following the CLSI guidelines. The pathogens were then screened for resistance genes including blaTEM, mcr-1, mecA, and blaNDM-1 genes. 

Results

Of 169 S. aureus isolates of clinical origin, 44.9% were methicillin-resistant S. aureus and 55 of them possessed the mecA gene. On the other hand, 46.7% of MRSA from water samples possess the mecA gene. The occurrence of the mcr-1 gene among E. coli was found to be 91.6% and 44% from clinical and poultry origins respectively. Most of E. coli were ESBL producers carrying 41.2% and 57.5% of the blaTEM gene from the clinical and water samples respectively. Similarly, P. aeruginosa from clinical origin were resistant to most of the antibiotics and 77.8% of MBL producers possess the blaNDM-1 gene. 

Conclusion

The resistant genes were ubiquitous in all sources. Hence, it is urgent to address one health concept and alert the policymakers to formulate strict antibiotic prescription policies in clinical settings and control the overuse of antibiotics in animal farming. 

SDG 3 targets:

3.D: Global health risks

Other SDGs:

SDG 3: Good health and well-being

 

Date and Place of Presentation: November 23, 2023, Thursday, Utrecht, Netherlands (1:30 PM Session at Social Impact Factory Building, Seoul).

Photograph 1: Presenting at ECTMIH2023

Photograph 2: ECTMIH Academy course