Antibiogram Profile of Respiratory Pathogens and Identifying Predisposing Factors of Respiratory Tract Infections among Patients Visiting Bhaktapur Hospital

Antibiogram Profile of Respiratory Pathogens and Identifying Predisposing Factors of Respiratory Tract Infections among Patients Visiting Bhaktapur Hospital

Shreemila Bajracharya1†, Jharana Thapa1†, Pushpa Thapa Magar1†, Ananda Kumar Mandal2, Niraj Manandhar2, Avinash Chaudhary1, Dinesh Dhakal1, Upendra Thapa Shrestha3*

 

¹Department of Microbiology, Sainik Awasiya Mahavidhyalaya, Bhaktpur, Nepal

²Bhaktapur Hospital, Bhaktapur, Nepal

³Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal

 

†These authors contributed equally.

 

*Corresponding author: Upendra Thapa Shrestha, Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal, E-mail: upendrats@gmail.com

 

ABSTRACT

 

Objectives: To address the current problem of MDR infections and respiratory bacterial pathogens and identify the risk factors associated with respiratory tract infections (RTIs).

Methods: A total of 327 RTI specimens were cultured using a conventional microbiological method to identify potential bacterial pathogens. The isolates were then subjected to antibiotic susceptibility testing using modified Kirby Bauer’s disc diffusion method. In addition, the risk factors associated with RTIs were obtained by direct interviews with patients using a structured questionnaire.

Results: The overall prevalence of RTIs among the study population was found to be 11.9%. The highest rate of infections was observed among patients of age group 61-70 years in both sexes and from ICU wards. Among the bacterial pathogens, Acinetobacter baumannii was isolated in the highest number followed by Pseudomonas aeruginosa. 64.2% A. baumannii isolates were resistant to Azithromycin but 100% sensitive to meropenem. P. aeruginosa resistance rate was 50% to Piperacillin-tazobactam, ciprofloxacin, Polymyxin B and Ceftriaxone, and had the highest MDR. Half of the Staphylococcus aureus isolates were MRSA. Risk factors such as previous infections, family history with RTIs, chronic use of antibiotics, and smoking were found to be significantly associated with RTIs.

Conclusion: We observed high MDR among the respiratory pathogens with growing resistance to β-lactam, macrolides, and Polymyxins whch necessitates alternative drugs in management. There is also need for targeted preventive strategies, prohibition of irrational use of antibiotics, and avoidance of exposure to risk factors such as smoking, outdoor air pollution, and chronic use of antibiotics.

 

Keywords: Respiratory tract infections, MDR, risk factors, Nepal

Citation: Bajracharya S, Thapa J, Thapa Magar P, Mandal AK, Manandhar N, Chaudhary A, Dhakal D, Thapa Shrestha U. Antibiogram profile of respiratory pathogens and identifying the predisposing factors of respiratory tract infections among the patients visiting Bhaktapur Hospital. TUJM, 2024; 11(1): 119-129.

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