Strong mosquitocidal Bacillus thuringiensis from Mt. Everest

(Published in Our Nature (2007)5: 67-69)

Strong mosquitocidal Bacillus thuringiensis from Mt. Everest

Upendra Thapa Shreshtha1, Gyan Sundar Sahukhal1, Kiran Babu Tiwari1, Subarna
Pokhrel2, Anjana Singh3, Vishwanath Prasad Agrawal4

1Research Laboratory for Biotechnology and Biochemistry (RLABB), Maitidevi, Kathmandu, Nepal;
2Enzyme Biotechnology Laboratory, Seoul National University, South Korea
3Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal
4 RLABB, Tel: +977-1-4442775, E-mail: vpa@wlink.com.np

Received: 21.10.2007, Accepted: 15.11.2007

Abstract
Bacillus thuringiensis strains were isolated from soil samples collected from Khumbu
Base Camp of the Everest region and characterized by standard microbiological
techniques viz. colonial and morphological characteristics, and biochemical tests. Insect
bioassay of each isolate was performed by standard method using mosquito larva. Among
ten randomly selected isolates, one isolate showed the highest insecticidal activity against
Dipteron insects.

Keywords: Insect-bioassay, Isolates, Khumbu region, Mosquitocidal, Mosquito larva

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