ABSTRACT PUBLISHED
ON
First
International Conference
Infectious
Diseases and Nanomedicine-2012 (ICIDN-2012)
December 15-18, 2012,
Kathmandu, NEPAL
Antibiogram and plasmid
profiling of multi drug resistant Escherichia coli
S Shrestha1,
NM Mali1, R Shrestha1, J Shrestha2, D Acharya1,
KB Tiwari1, N Adhikari1, UT Shrestha1
1: Kantipur College of Medical Science, Tribhuvan University,
Sitapaila, Kathmandu, Nepal
2: Walter Reeds/ AFRIMS Research Unit Nepal (WARUN), Kathmandu,
Nepal
Multiple antibiotic resistances among
common bacterial pathogens have been established as an alarming public health
problem elsewhere. This study was aimed to explore prevalence of
Extended-spectrum beta-lactamase (ESBL) producers among multi-drug resistant
(MDR) and plasmid profiling patterns of Escherichia coli isolated in Kathmandu
Model hospital, Nepal over three months period. Altogether 49 isolates were
randomly selected among the reported MDR E.
coli and retested for their susceptibility pattern and ESBL production by
Kirby-Bauer disc diffusion test. All isolates were completely resistant to
Amoxycillin but sensitive to Imipenem. A very high prevalence (44, 89.8%) of
ESBL producers was detected. Most of the isolates were resistant to commonly
used antibiotics such as quinolones, tetracyclines and Cotrimoxazole. Among
quinolones, the Minimum Inhibitory Concentration (MIC) range for Nalidixic
acid, Ciprofloxacin and Ofloxacin were 512 - >4093, 8 - 4096 and 1 - 256
µg/ml respectively. All 31 (63.3%) plasmid-harboring isolates contained a
>33.5 kb sized plasmid. Among them, seven isolates possessed multiple (2 –
7) plasmids. Overall, twelve different resistance patterns were observed among
the bacteria. Based on the patterns, the high molecular weight plasmid seemed
to contain most of the resistance genes. It is suspected that multi drug
resistance and ESBL production in E. coli
with resistance to quinolones may be due to their high molecular weight
plasmids. So, continuous antibiotic susceptibility test and surveillance of the
plasmid and chromosome of E. coli is
essential as plasmid analysis has been applied to determine the evolution and
spread of antibiotic resistance among isolates.
KEYWORDS: Escherichia coli, Antibiotic resistance
pattern, Multidrug resistant (MDR),
Extended spectrum Beta-lactamases (ESBL), Plasmid profiling
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