A Practical Manual for Microbial Genetics

New Book on Practical Microbial Genetics

TABLE OF CONTENTS

Contents
Orientation of Microbial genetics / Molecular Biology Laboratory Theory Micropipettes DNA Extraction Role of Sodium in DNA Extraction Restriction Enzymes Plasmid Bacteriophages Polymerase Chain Reaction-PCR
Experiment no. 1	Calibration of Micropipettes
Experiment no. 2	Preparation of different Tris buffers: Tris-HCl, Trsi-EDTA and TAE
Experiment no. 3	Isolation of DNA from human blood
Experiment no. 4a	Extraction of chromosomal DNA from Gram positive bacteria
Experiment no. 4b	Extraction of chromosomal DNA from Gram negative bacteria
Experiment no. 5a	Plasmid DNA extraction from bacteria (Alkaline lysis method)
Experiment no. 5b	Isolation and purification of bacterial plasmid with Qiagen spin (QIAprep) Miniprep system
Experiment no. 6	RNA extraction from yeast
Experiment no. 7	Spectrophotometric analysis of DNA in extracted solution
Experiment no. 8	Agarose gel electrophoresis of DNA
Experiment no. 9a	Demonstration of induction / repression mode of lac-operon regulation system in Escherichia coli
Experiment no. 9b	Determination of level of induction of lac-operon in Escherichia coli
Experiment no. 10	Conjugation in bacteria: Co-culture of MDR Salmonella enteritidisTyphi with Escherichia coli HB 101 and detection of transconjugants by antibiotic selection method
Experiment no. 11	Transformation of Escherichia coli HB101 by plasmids: pUC18 or pBR322
Experiment no. 12	Study of performance of HindIII and EcoRI restriction enzymes
Experiment no. 13a	Construction of recombinant DNA: Preparation and purification of insert (Lambda DNA fragments) and vector (pUC18) DNA
Experiment no.13b	Construction of recombinant DNA: Ligation of purified insert (Lambda DNA fragments) and vector (pUC18) DNA
Experiment no. 14a	Amplification of recombinant DNA: Transformation of recombinant pUC18 DNA in Escherichia coli HB101
Experiment no.14b	Detection of recombinant DNA: Extraction of recombinant pUC18 DNA from transformed Escherichia coli HB101 clones
Experiment no. 14c	Detection of recombinant DNA: Extraction of recombinant pUC18 DNA in plasmid extracts of transformed Escherichia coli HB101 clones by agarose gel electrophoresis
Experiment no. 15a	Amplification of Escherichia coli DNA (genes) by Polymerase chain Reaction (PCR)
Experiment no.15b	Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)