SCIENTIFIC DEDICATION

“I will educate my kids whether I have to sell my eyes or any other organs”. It is not a proverb by a renowned educationist or by any popular artist. It is a true speaking of my father, Ratna Keswor Thapa Shrestha, born in Bode, Bhaktapur, Nepal on April 15, 1936. Due to poor financial condition of my great-father, my father could not get even primary level education. He had just gone a local school in Bode for a month and studied the First Book (named at that time). I was extremely surprised about his knowledge that he could teach us even a secondary level courses especially on Mathematics and Science subjects. He had a strong desire of learning himself and teaching his kids. I had never seen him wasting his time. I always saw him with some kinds of books and newpapers especially health related books at his last moments.  With his enormous desire, he did all his best to educate his nine kids (six sons and three daughters) including myself (the youngest one). One can imagine the life of a poor joint family is a difficult one. He was finally succeeded to provide us good education with his small local curd business. Under bleak conditions, my parents left no stones unturned to get me a good education and my elder brothers added on his effort.  Now, I am M.Sc. graduate in Microbiology and recently selected as lecturer in Tribhuvan University. I have been awarded with few Research Grants from National and International Institutions, published dozens of research articles, written three books and many other achievements in Jobs, which wouldn’t be possible without the effort of my Father. He is no more with us (Expired on May 22, 2017 Monday at the age of 82). No matter where he is, he is always present in my heart and he watching us from Heaven.

I feel really lucky and blessed to have a father like him. He was true motivation in my life, just seeing his face make my day amazing.  Even I spent a million of words on his goodwill, will not be enough.

I wish for his soul to be calm and peaceful!!!!

Finally, from the depth of my heart I would like to dedicate all my achievements to my beloved FATHER.

I LOVE YOU, DAD

MY ACHIEVEMENTS (DEDICATED TO MY FATHER)

  

BOOKS PUBLISHED

1.       Shrestha UT, Adhikari N and Shrestha S (2014). A Practical Manual for Biochemistry and Instrumentation, Second Edition, Oasis Publication, Anamnagar, Kathmandu, Nepal (ISSn No.: 978-9937-2-7961-1).

2.       Shrestha UT and Adhikari N (2014). A Practical Manual for Microbial Genetics, First Edition, Publisher-Kantipur College of Medical Science (ISSN No: 978-9937-2-7962-8).

3.       Shrestha UT, Adhikari N and Shrestha S (2012). A Practical Manual for Biochemistry and Instrumentation, First Edition, Oasis Publication, Anamnagar, Kathmandu, Nepal.

4.       Shrestha UT (2010) A Practical Manual of Environmental Microbiology, Khwopa College Publisher, Bhaktapur, Nepal.

 PUBLISHED ORIGINAL ARTICLES

1. Prakash Simkhada, Shiva Raj K. C., Santosh Lamichhane, Surya Subedi & Upendra Th apa Shrestha Bacteriological Profile and Antibiotic Susceptibility Pattern of Blood Culture Isolates from Patients Visiting Tertiary Care Hospital in Kathmandu, Nepal. Global Journal of Medical Research: © Microbiology and Pathology; Volume 16 Issue 1 Version 1.0 Year 2016; 24-31.
2. Sabnum Shrestha, Sulochana Laluwa, Bimala Ranamagar, Pramila Thapa, Manoj Pradhan, Nabaraj Adhikari, Upendra Thapa Shrestha (2016). Intestinal Parasitosis among School Going Children in Bhaktapur, Nepal. International Journal of Medicine and Biomedical Sciences, 1(2); 1-6.
3. Bhomi Ujwol, Rijal Komal Raj, Neupane Biswas, Shrestha Santu, Chaudhary Mahesh, Acharya Dhiraj, Thapa Shrestha Upendra, Adhikari Nabaraj and Ghimire Prakash (2015).  Status of inducible clindamycin resistance among macrolide resistant Staphylococcus aureus. African Journal of Microbiology Research. Vol 10(9); 280-284 (March 7 2016).
4. Rojina Maharjan, Dina Shrestha, Jyoti Acharya, Nabaraj Adhikari, Komal Raj Rijal, Prakash Ghimire, Upendra Thapa Shrestha (2015). Change in Biotype trend of Vibrio cholerae in Nepal.  Med-Micro Journal of Microbiology (MMJM), 1 (1): 45-54.
5. Upendra Thapa Shrestha, Nabaraj Adhikari, Rojina Maharajan, Megha R Banjara, Komal R Rijal, Shital R Basnet, Vishwanath P Agrawal. Multidrug resistant Vibrio cholerae O1 from clinical and environmental samples in Kathmandu city. BMC Infectious Diseases, 2015; 15: 104. http://www.biomedcentral.com/orderreprints/s12879-015-0844-9 (IMPACT FACTOR-2.61).
6. Khanal S, Ansari S, Basnyat SR, Joshi DR, Adhikari N, Shrestha UT, Acharya D, Adhikari P, Niraula PM, Khadka R and Upadhyay BP (2015). Sero-prevalence of Japanese Encephalitis (JE) among Nepalese Children. British Microbiology Research Journal, 6(3): 126-134, 2015, Article no.BMRJ.2015.065.

7.       Prakash Chandra Pahadi, Upendra Thapa Shrestha, Nabaraj Adhikari, Pradeep Kumar Shah and Ritu Amatya (2014). Growing resistance to Vancomycin among Methicillin Resistant Staphylococcus aureus isolates from different clinical samples. Journal of Nepal Medical Association (JNMA), Vol 52 no.12, Issue 196 (Oct-Dec), 977-981.

8.       Adhikari N, Shrestha UT, Shah PK, Khadka R, Shrestha S, Khanal S, Adhikari P, Shrestha S, Mali NM, Basnyat SR (2014). Intestinal parasitic infection among school children in Kathmandu Valley. Tribhuvan University Journal of Microbiology (TUJM), Vol. 2, No. 1, 6-9.

9.       ShresthaUT, Agrawal VP (2014). Survival and efficiency of Bacillus thuringiensis in waste water for biological control of mosquito breeding. The Journal of University Grants Commission, Vol. 3, No. 1, 134-139.

10.    Shrestha S, Mali NM, Tiwari KB, Adhikari N, Shrestha UT, Basnyat SR (2014). Antibiogram and Plasmid profiling of clinical multidrug resistant Escherichia coli. Journal of Institute of Medicine, August Vol-36(2): 21-26 (Website: www.jiom.com.np).

11.    Shrestha UT, Sujakhu H (2014). Coliform and Vibrio cholerae analysis of drinking water collected from cholera outbreak region of Bhaktapur Municipality. International Journal of Environment, Jun-Aug 2014 vol 3 (3): 39-45(Online: http://www.nepjol.info/index.php/IJE/index).

12.    Adhikari N, Shrestha UT, Shah PK, Khadka R, Shrestha S, Khanal S, Adhikari P, Shrestha S, Mali NM, Basnyat SR (2014). Intestinal parasitic infection among school children in Kathmandu valley. Tribhuvan University Journal of Microbiology, vol 2 (1): 6-9.

13.    Shrestha UT (August 2013). Nepali pig farmers learn management techniques from TIRI scholar. http://lccrsp.org/2013/08/researchers-make-significant-improvements-to-Nepal-pig-farmers-practices/ (Official website of Feed and Future Innovative lab Collaborative research, Colorado State University).

14.    Shikshya Gautam, Pradeep Kumar Shah, Nabaraj Dahal, Dhiraj Acharya, Nabaraj Adhikari, Upendra Thapa Shrestha, and Susma Thapa Magar (2012). Multidrug resistant bacterial pathogens among patients visiting a tertiary care hospital in Central Nepal. Procedding of First International Conference on Infectious Diseeases and Nanomedicine, December 15-18, 2012: 7-13.

15.    Tiwari KB and Shrestha UT. Nitrogen Assimilation in Actinomycetes. Nepalese Journal of Microbiology (NJM), Vol 2, No 1, 2011, pp.61-65

16.    Khatri M, Rajbhandari Y, Regmi S, Hamal J, Shrestha UT and Kiran Babu Tiwari KB¹, Sodium azide induced mutation of actinomycetes  International Journal of Life Sciences ISSN 2091-0525, vol. 3, 2009  http://www.nepjol.info/index.php/IJLS/.

17.    Sahukhal GS, Shrestha UT, Lekhak B, Singh A and Agrawal VP; Genetic Fingerprinting of Bacillus thuringiensis isolates by Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). Nepal Journal of Science and Technology 10 (2009) 97-103.

18.    Sahukhal GS, Jayana BL, Shrestha UT, Ben-Dov E and Agrawal VP; Screening of cry-type genes among Bacillus thuringiensis isolated from soil samples in Phereche and Sagarmatha National Park of Mount Everest base camp region by PCR. J. Food Sci. & Technol. Nepal, ISSN: 1816-0727, 2008; vol  4, 74-77.

19.    Yadav J, Shrestha UT, Tiwari KB, Sahukhal GS and Agrawal VP. Streptomycin – like antibiotic from Streptomyces species isolated from Mount Everest Base Camp Nepal. Journal of Science and Technology 9(2008) 73-75).

20.    Tiwari KB, Shrestha UT, Acharya A, Subedi B, Paudyal B, Jnawali M, Shakya P, K.C. U, Singh A and Agrawal VP; Antibacterial activities of locally used toothpastes against dental pathogens. Journal of Institute of Medicine, August, 2008; 30:2 15-18.

21.    Sahukhal GS, Shrestha UT,  Tiwari KB, Lekhak B, Singh A and Agrawal VP; Optimization of RAPD-PCR for Genetic Fingerprinting of Bacillus thuringiensis. Journal of Institute of Science and Technology, Vol. 15, 2007-08, pp.90-102.

22.    Shah SK, Tiwari KB, Shrestha UT, Pokhrel S and Agrawal VP; Cloning of Bacillus thuringiensis cry3 fragment in Escherichia coli Journal of Institute of Science and Technology, Vol. 15, 2007-08, pp.64-70.

23.    Dhungel B, Subedi M, Tiwari KB, Shrestha UT, Pokhrel S and Agrawal VP; Thermostable Glucose Isomerase from Psychrotolerant Streptomyces species International Journal of Life Sciences (2007) vol-1,  http://www.nepjol.info/index.php/IJLS/.

24.    Shrestha UT, Sahukhal GS, Pokharel S, Tiwari KB, Singh A and Agrawal VP; Strong mosquitocidal Bacillus thuringiensis from Mount Everest (2007) Our Nature 5:67-69.

25.    Shrestha UT, Sahukhal GS, Pokharel S, Tiwari KB, Singh A and Agrawal VP; Delta Endotoxin Immunocrossreactivity of Bacillus thuringiensis isolates collected from Khumbu Base Camp of Mount Everest Region. (2006) J. Food Sci. & Technol. Nepal 2:128-131.

GRANTS AWARDED

1. USAID Grant: Colorado State University, Feed the Future Innovation Lab; Increase in milk production among dairy cattle after deworming of gastrointestinal parasites, 2015 (as Principle Investigator-TIRI Scholar) Second Project.
2. UGC (University Grants Commission, Nepal) grants: Faculty Research Grants for the project entitled "Antibiogram and detection of ctx gene in Vibrio cholerae from clinical and environmental specimens by specific PCR for Molecular diagnosis", 2013 (as Team Leader).
3. USAID Grant: Colorado State University, Feed the Future Innovation Lab; Comparative Study of parasitic infection in Pig Population from Different climatic ecozones of Nepal and Its impact on productivity and human health, 2013 (as Principle Investigator-TIRI Scholar), First Project.
4. UGC (University Grants Commission, Nepal) grants: Mini Research Grants for research project entitled "Study of survivability and efficiency of Bacillus thuringiensis   in waste water for biological control of mosquito breeding", 2010 (as Principle Investigator).

ARTICLES REVIEW FOR INTERNATIONAL JOURNAL

1. Cholera outbreak caused by drug resistant Vibrio cholerae serogroups O1 biotype ElTor serotype Ogawa in Nepal; a cross sectional study. Antimicrobial Resistance and Infection Control, BioMed Central, www.biomedcentral.com (An impact factor Journal).

WHO Projects

1. Situation Analysis of Malaria in cross-border settings of Nepal: supported by WHO country office Nepal in collaboration with EDCD and KCMS, 2016 (as a research officer).
2. Development guideline and SOPs for Malaria Case based Surveillance and Implementation plan with year wise budgeting for NMSP 2014-2025. supported by WHO country office Nepal in collaboration with EDCD and KCMS, 2015
3. Assessment of laboratory capacity for malaria microscopy in health facilities of malaria endemic districts in Nepal: supported by WHO country office Nepal in collaboration with EDCD and KCMS, 2015 (as a research assistant).
4. Assessment of impact of the OFID support in strengthening blood transfusion service in Nepal: supported by Ministry of Health and Population, Government of Nepal, WHO, OFID and Nepal Red Cross Society, 2015 (as a research assistant).
5. Monitoring the durability of Long Lasting Insecticidal Nets (LLINs) in the field condition of Nepal, 15 Nov to 25 Dec, 2013. WHO project for malaria eradication (As a research assistant).

PRESENTATIONS

1.        Comparative study of parasitic infection in pig population from different climatic ecozones of Nepal and its impact on productivity and human health. The Fifth National Conference on Microbiology (5^th NCM) organized by Nepalese Society for Microbiology (NESOM), on Nov15-Nov16, 2014, Dhobighat, Kathmandu pp 99-100.

2.        Multidrug resistant Vibrio cholerae O1 and detection of ctx gene from clinical and environmental specimens in Kathmandu Valley. The Fifth National Conference on Microbiology (5^th NCM) organized by Nepalese Society for Microbiology (NESOM), on Nov15-Nov16, 2014, Dhobighat, Kathmandu.

3.        Study of survivability and efficiency of Bacillus thuringiensis in waste water for biological control of mosquito breeding. The Sixth National Conference on Science and Technology, September. 25-27, 2012, Kathmandu pp. 79.

4.        Delta Endotoxin Diversity among Cold Tolerant Bacillus thuringiensis from Khumbu, Everest Base Camp. The Fifth National Conference on Science and Technology, Nov. 10-12, Kathmandu pp. 184-185.