(Report to be submitted at UGC)
ABSTRACT
Bacillus thuringiensis strains were isolated from soil samples collected from Sagarmatha National Part of the Everest region and subsequently identified by standard microbiological techniques including colonial characteristics, morphological characteristics and biochemical characteristics. Some of potent strains already preserved in lab were also revived and recharacterized for their crystal protein production property. The stationary phase culture broth was tested for insect bioassay. All these isolates were able to kill the mosquito larva even at their natural breeding place, waste water and were further used for survivability test in the waste water. The growth of B. thuringiensis in waste water was found to be soared for seventh days and plunged gradually in subsequent days till 30 days. The survivability of B. thuringiensis isolates was recorded till 30 days and found to be alive in the waste water. The number of B. thuringiensis was found to be higher in control in compare with the test, unautoclaved waste water.
