Friday, January 24, 2020

For Awareness purpose on Novel Coronavirus



Novel Coronavirus
(Knowledge to share about an emerging infectious diseases from CDC)
2019 Novel Coronavirus (2019-nCoV) is a virus (more specifically, a coronavirus) identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China. Early on, many of the patients in the outbreak in Wuhan, China reportedly had some link to a large seafood and animal market, suggesting animal-to-person spread. However, a growing number of patients reportedly have not had exposure to animal markets, suggesting person-to-person spread is occurring. At this time, it’s unclear how easily or sustainably this virus is spreading between people.  The latest situation summary updates are available on CDC’s web page 2019 Novel Coronavirus, Wuhan, China.

Symptoms
Patients with confirmed 2019-nCoV infection have reportedly had mild to severe respiratory illness with symptoms of:
  • fever
  • cough
  • shortness of breath
CDC believes at this time that symptoms of 2019-nCoV may appear in as few as 2 days or as long as 14 after exposure. This is based on what has been seen previously as the incubation period of MERS viruses.
The latest situation summary updates are available on CDC’s web page 2019 Novel Coronavirus, Wuhan, China.

How it Spreads

Coronaviruses are a large family of viruses that are common in many different species of animals, including camels, cattle, cats, and bats. Rarely, animal coronaviruses can infect people and then spread between people such as with MERS and SARS. Many of the patients in the pneumonia outbreak caused by 2019-nCov in Wuhan, China had some link to a large seafood and live animal market, suggesting animal-to-person spread. However, a growing number of patients reportedly have not had exposure to animal markets, indicating person-to-person spread is occurring.
When person-to-person spread has occurred with MERS and SARS, it is thought to have happened via respiratory droplets produced when an infected person coughs or sneezes, similar to how influenza and other respiratory pathogens spread. Spread of SARS and MERS between people has generally occurred between close contacts.
It’s important to note that how easily a virus spreads person-to-person can vary. Some viruses are highly contagious (like measles), while other viruses are less so. It’s not clear yet how easily 2019-nCoV spreads from person-to-person. It’s important to know this in order to better understand the risk associated with this virus.
There is much more to learn about the transmissibility, severity, and other features associated with 2019-nCoV and investigations are ongoing. The latest situation summary updates are available on CDC’s web page 2019 Novel Coronavirus, Wuhan, China.

Prevention & Treatment

Prevention

There is currently no vaccine to prevent 2019-nCoV infection. The best way to prevent infection is to avoid being exposed to this virus. Right now, 2019-nCoV has not been found to be spreading in the United States, so there are no additional precautions recommended for the general public to take. However, as a reminder, CDC always recommends everyday preventive actions to help prevent the spread of respiratory viruses, including:
  • Wash your hands often with soap and water for at least 20 seconds. If soap and water are not available, use an alcohol-based hand sanitizer.
  • Avoid touching your eyes, nose, and mouth with unwashed hands.
  • Avoid close contact with people who are sick.
  • Stay home when you are sick.
  • Cover your cough or sneeze with a tissue, then throw the tissue in the trash.
  • Clean and disinfect frequently touched objects and surfaces.
These are every day habits that can help prevent the spread of several viruses. CDC does have specific guidance for travelers.

Treatment

There is no specific antiviral treatment recommended for 2019-nCoV infection. People infected with 2019-nCoV should receive supportive care to help relieve symptoms. For severe cases, treatment should include care to support vital organ functions.
People who think they may have been exposed to 2019-nCoV should contact your healthcare provider immediately.


Sunday, January 12, 2020

Bacterial Profile and Their Antibiogram Isolated from Cell Phones


Bikrant Gumanju1, Roshna Shrestha1, Poonam Lakhemaru1, Rakysa Upadhyaya1, Sushila Shrestha1, Dinesh Dhakal1, Upendra Thapa Shrestha2*
1Department of Microbiology, Sainik Awasiya Mahavidhyalaya, Sallaghari, Bhaktapur
2Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathamandu

*Corresponding author: Upendra Thapa Shrestha, Assistant Professor, Central Department of
Microbiology, Tribhuvan University, Kirtipur, Kathmandu, E-mail: upendrats@gmail.com

ABSTRACT
Objectives: The present study aimed to identify bacteria profile of cell phones used by different people from different profession of Bhaktapur and to access their antimicrobial resistance.
Methods: Forty-nine mobile swab samples were collected from 7 different profession category (7 samples each from student, butcher, cook, panipuri vendor, health workers, and dairy employee). Samples were cultured and processed by standard Microbiological procedures. All the isolates were further subjected to antibiotic susceptibility testing using modified Kirby Bauer disc diffusion method as describe in CLSI guidelines. The rate of multiple drug resistant (MDR) bacteria was also determined.
Results: Out of 49 sample, Bacillus spp (20.4%) was the most predominant isolate, followed by
Staphylococcus aureus (10.6%) and Pseudomonas spp (10.6%). Higher variety of bacterial isolates was found in the cell phones swabs from butcher followed by cook, farmer and panipuri vendor group. The data from the questionnaire showed that handkerchief or tissue paper were mostly used by respondents to clean their mobile. All Gram-positive and Gram-negative isolates were resistance to Cefoxitin (100%) except Micrococcus spp and Neisseria spp. Gram positive (18.2%) and Gram-negative (36.9%) isolates were identified as MDR. All S. aureus and coagulase negative staphylococci were methicillin resistant
Conclusion: The cell phones of people from different profession were found to possess many different bacterial pathogens including multi drug resistant strains which could be the possible pathogens for food borne infections and opportunistic infections.
Key words: Cell phones, Antimicrobial susceptibility test, MDR, Standard microbiological procedure
Date of Submission: October 3, 2019            
Date of Acceptance: November 27, 2019
Published Online: December, 2019               
DOI: https://doi.org/10.3126/tujm.v6i0.26591

Citation: Gumanju et al. 2019, Bacterial Profile and Their Antibiogram Isolated from Cell Phones TUJM 6(1): 96-102

Effect of deworming on milk production in dairy cattle and buffaloes infected with gastrointestinal parasites in the Kavrepalanchowk district of central Nepal

Upendra Thapa Shrestha1,2,3, Nabaraj Adhikari1,3, Samarpan Kafle3, Nabaraj Shrestha4, Megha Raj Banjara1, Katie Steneroden5, Richard Bowen5, Komal Raj Rijal1‍, Bipin Adhikari6, Prakash Ghimire1

1Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal
2Research Laboratory for Biotechnology and Biochemistry, Sanepa, Lalitpur, Nepal
3Department of Microbiology, Kantipur College of Medical Science, Sitapaila, Nepal
4Regional Directorates of Livestock Services, Birendranagar, Nepal
5Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA
6Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol
University, Bangkok, Thailand

ABSTRACT

Background: In Nepal, knowledge of proper handling, management and causes of cattle diseases is still limited. The main objective of this study was to explore the impact of deworming on milk production and its effect on milk qualities. 
Methods: A total of 200 faecal samples (100 buffaloes and 100 cows) were collected and analysed for parasitic burden. Half of the infected cattle (buffaloes, Bos bubalis; cow native, B. indicus; European, B. taurus) were then dewormed with Levamisole Hydrochloride-Oxyclozanide bolus, and the remaining 50 per cent were left untreated. The milk yield from both infected and dewormed cattle was recorded for 30 days and the qualities of milk were analysed.
Results: The prevalence of parasitic infection was found to be 22.0 per cent. Fasciola hepatica was the predominant parasite (81.8 per cent), followed by Toxocara vitulorum (34.1 per cent), Strongyloides papillosus (6.8 per cent) and Bunostomum phlebotomum (4.5 per cent). The average
milk yield (litre/day/cow) significantly increased, which was 1.22 litres per day for treated cows and 1.06 litres for treated buffaloes. The intervention effect of deworming among cows was 0.79 (14.06 per cent increment) and for buffaloes was 0.42 (8.32 per cent increment). After deworming the infected cattle, the protein percentage was significantly improved in cows (P=0.035), whereas the lactose percentage and solid percentage had increased significantly in buffaloes (P=0.002 and P=0.028). 
Conclusion: Antiparasitic treatment in cattle had positive effects on milk qualities such as solid non-fat, lactose, solid percentage and total protein percentage.

Citation: Thapa Shrestha U, Adhikari N, Kafle S, et al. Effect of deworming on milk production in dairy cattle and buffaloes infected with gastrointestinal parasites in the Kavrepalanchowk district of central Nepal. Veterinary Record Open 2020;7:e000380. doi:10.1136/ vetreco-2019-000380
Received 13 November 2019 Accepted 27 November 2019

Thapa Shrestha U, et al. Vet Rec Open 2020;7:e000380. doi:10.1136/vetreco-2019-000380

Bacteria in Photos

Bacteria in Photos