Manuscript accepted in TUJM

Microbial quality analysis of panipuri samples collected from different parts of Bhaktapur

 Punam Ghimire¹, Sujata Khand¹, Bhawana Chaulagain¹, Ashish Siwakoti¹, Dinesh Dhakal¹, Upendra Thapa Shrestha² *

¹Sainik Awasiya Mahavidhyalaya (affiliated to Tribhuvan University) Sallaghari, Bhaktapur

²Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal

 

*Corresponding Author: Upendra Thapa Shrestha, Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Email: upendra.thapashrestha@cdmi.tu.edu.np / upendrats@gmail.com

ABSTRACT

Objectives: The study was aimed to identify potential bacterial pathogens from the panipuri samples collected from different vendors of Bhaktapur district and determine their antibiogram patterns.

Methods: Altogether 120 (40 puri, 40 stuffing and 40 pani) samples of panipuri were collected from different vendors of Bhaktapur district in a cold chain and were transported to the microbiology laboratory. Puri and stuffing were then pre-enriched, enriched and cultured while pani samples were processed using the MPN method. All the isolates were identified following standard microbiological procedure and subjected to antibiotic susceptibility testing following CLSI guidelines.

Results: The result revealed contamination of 77.5% stuffing, 67.5% puri and 52.5%  pani samples with pathogenic bacteria. Among bacterial pathogens, the highest number was Staphylococcus aureus followed by Escherichia coli and Salmonella spp. 100% of S. aureus were found to be resistant to ampicillin and novobiocin. Similarly, 94.1% of E. coli were resistant to ampicillin followed by ciprofloxacin (64.7%). A very few isolates of Salmonella spp, Shigella spp and Vibrio spp were resistant to tetracycline. The highest number of multidrug-resistant bacteria were S. aureus, followed by Klebsiella spp and E. coli.

Conclusion: The study showed that the panipuri samples from street vendors were found to be highly contaminated with pathogenic bacteria which might affect consumers’ health. Thus, to prevent any food-borne illness in the future, frequent evaluation and regulation of the quality of such foods should be carried out.

Keywords: Street foods, panipuri, bacterial pathogens, antimicrobial-resistant, multidrug-resistant

Vaccine updates for COVID-19

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There are several COVID-19 vaccines validated for use by WHO (given Emergency Use Listing). The first mass vaccination programme started in early December 2020 and the number of vaccination doses administered is updated on a daily basis on the COVID-19 dashboard.

The WHO Emergency Use Listing process determines whether a product can be recommended for use based on all the available data on safety and efficacy and on its suitability in low- and middle-income countries. Vaccines are assessed to ensure they meet acceptable standards of quality, safety and efficacy using clinical trial data, manufacturing and quality control processes. The assessment weighs the threat posed by the emergency as well as the benefit that would accrue from the use of the product against any potential risks.

In line with their national regulations and legislation, countries have the autonomy to issue emergency use authorizations for any health product.  Domestic emergency use authorizations are issued at the discretion of countries and not subject to WHO approval.

As of 12 January 2022, the following vaccines have obtained EUL:

• The Pfizer/BioNTech Comirnaty vaccine, 31 December 2020.
• The SII/COVISHIELD and AstraZeneca/AZD1222 vaccines, 16 February 2021.
• The Janssen/Ad26.COV 2.S vaccine developed by Johnson & Johnson, 12 March 2021.
• The Moderna COVID-19 vaccine (mRNA 1273), 30 April 2021.
• The Sinopharm COVID-19 vaccine, 7 May 2021.
• The Sinovac-CoronaVac vaccine, 1 June 2021.
• The Bharat Biotech BBV152 COVAXIN vaccine, 3 November 2021. 
• The Covovax (NVX-CoV2373) vaccine, 17 December 2021.
• The Nuvaxovid (NVX-CoV2373) vaccine, 20 December 2021

Reference: https://www.who.int/news-room/questions-and-answers/item/coronavirus-disease-(covid-19)-vaccines

Journal of Nepal Health Research Council (JNHRC)

 

Higher rate of extreme drug-resistant Klebsiella pneumoniae infections among cardiac patients attending a Tertiary Care Hospital in Kathmandu

Subash Paudel¹, Pradeep Kumar Shah¹, Prashanna Adhikari¹, Sanjay Singh K.C.², Upendra Thapa Shrestha³*

¹Department of Microbiology, Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu

²Department of Pathology, Sahid Gangalal National Heart Center, Bansbari, Kathmandu

³Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu

* ^­Corresponding author: Upendra Thapa Shrestha, Central Department of Microbiology, Tribhuvan University, Kiritpur, Kathnamdu, Nepal. Email: upendrats@gmail.com

ABSTRACT

Background: The accelerating rate of carbapenems resistance in Klebseilla pneumoniae isolates has put the treatment option worrisome. The effective strategy to ameliorate this alarming situation is possible through enhancing the combination therapy and appropriate laboratory diagnosis. Hence, the study was focused on identifying carbapenemase-producing K. pneumoniae and their antibiogram pattern.

Methods: A total of 944 clinical samples from patients attending Sahid Gangalal National Heart Center were processed from September 2019 to March 2020 to identify the possible bacterial pathogens following the standard microbiological procedures. K. pneumonaie isolates were further subjected to antibiotic susceptibility testing by the modified Kirby Bauer disc diffusion technique. Phenotypic confirmation of carbapenemase production was done by the modified carbapenemase inactivation method. The minimum inhibitory concentration of colistin was determined by the broth microdilution method.

Results: Of the total 944 samples, 15.47% (146) samples showed bacterial growth, among which 23.97% (35) were K. pneumoniae. Out of 35 K. pneumoniae isolates, 45.71% (16) were multidrug-resistant followed by 42.86% (15) extensively drug-resistant. Fourteen isolates of K. pneumoniae were carbapenemase producers among which 20% (7) were  serine carbapenemase while 20% (7) showed metallo-β-lactamase production. All the carbapenemase-producing K. pneumoniae were susceptible to colistin with <0.125µg/ml. Carbapenemase activity showed statistically significant with multidrug resistance (p=<0.05).

Conclusions: An increasing resistance to the carbapenem drugs showed a great problem in the management of   K. pneumoniae infections among immunocompromised patients especially cardiac patients however, colistin can be still an ultimate choice of drug for disease management.

Keywords: Klebsiella pneumoniae; Extreme Drug-Resistant; Carbapenemase; Broth Micro Dilution; Colistin;

 

Citation: Paudel et al., 2021. Higher rate of extreme drug-resistant Klebsiella pneumoniae infections among cardiac patients attending a Tertiary Care Hospital in Kathmandu J Nepal Health Res Counc 2021 Oct-Dec; 19 (53): 661-8.

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