Preface
Forward
Acknowledgements
List of Abbreviations
List of Tables
Lists of Figures
Format
Orientation
on Instrumentation
Basic
Techniques
Experiment no.
1 Preparation of different types of solutions (Percent, Molar, Normal,
Solutions from Solutions and Titrations, Concentrated Solutions Saturated) used
in biochemistry lab
Experiment
no. 2 Preparation of different types of
Buffers used in biochemical Studies
Experiment no.
3 Determination of lmax of different colored and noncolored compounds by
spectrophotometer
Experiment
no. 4 Verification of validity of Beer’s law and determination of the
molar extinction coefficient of BSA
Qualitative
Methods
Experiment no.
5 Qualitative detection of proteins and amino acids
Experiment
no. 6 Qualitative detection
of carbohydrates
Experiment
no. 7 Identification
of the nature of
carbohydrate in a given sample
Isolation and
Quantitative Methods
Experiment no. 8 Titration curve for the given amino acids
Experiment
no. 9
A. Quantitative determination of free amino acid content in serum
B.
Comparative study of free amino
acid content in germinating and non-germinating seeds
Experiment no. 10 Determination
of Tyrosine content in a given solution using standard calibration curve
Experiment no. 11 Quantitative determination of protein
A.
Biuret method
B.
Bradford method
C.
Folin-Lowry method
D.
UV method
Experiment no. 12 Isolation
of Casein from milk
Experiment no. 13 Isolation and assay of glycogen from the liver and skeletal muscle
Experiment
no. 14 Benedict’s test for reducing sugars
Experiment no. 15 Determination of soluble carbohydrates by Anthrone Method
Experiment no: 16 Determination of acid value of the given fats and oils
Experiment no: 17 Determination of the Iodine Number of lipids
Experiment no. 18 Quantitative analysis of DNA by flourimetry
Purification
and Identification Methods
Experiment no.
19 Preparation of various
sub-cellular fractions of liver cells by Differential Centrifugation method
Experiment
no. 20 Fractionation of serum protein by salt (Sodium chloride and
Ammonium sulphate to separate of albumin and globulin)
Experiment
no. 21 Precipitation and separation of
protein of interest by TCA (Trichloroacetic acid) method from biological sample
Experiment
no. 22 Separation and identification of amino acids in a given mixture by
ascending paper chromatography
Experiment
no. 23 Separation and identification of amino acids in a given mixture by
two dimensional paper chromatography
Experiment no. 24 Separation and identification of sugars (sugar juices) by
adsorption Thin layer chromatography (TLC)
Experiment no. 25 Extraction
and identification of lipids from given biological samples by Thin layer
chromatography
Experiment no. 26 Purification of proteins by size exclusion chromatography (gel
filtration)
Experiment no. 27 Separation of serum proteins by Ion-exchange Chromatography using
cation exchanger
Experiment no. 28 Analysis of serum albumin by affinity chromatography using Affi-Gel
Blue
Experiment no. 29 Analysis of serum protein by electrophoresis using cellulose
acetate paper
Experiment no. 30 Determination of molecular weight of a given protein by SDS-PAGE
(Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis)
Experiment no. 31 Native PAGE (Poly Acrylamide Gel Electrophoresis) of proteins
Experiment no. 32 Determination of molecular weight of DNA (genomic and plasmid) by
Agarose gel electrophoresis
Experiment no. 33 Detection of proteins and DNA in gels by Silver Staining
Clinical
Biochemistry
Experiment
no. 34 Determination of blood glucose
Experiment no. 35 Quantitative determination of total protein in serum and plasma
Experiment no. 36 Determination of Albumin in serum or plasma
Experiment no. 37 Determination of Direct and Total Bilirubin in serum
Experiment no. 38 Determination of hemoglobin by cyanmethemoglobin method
Experiment no. 39 Determination of SGOT (ASAT) activity in serum
A.
Colorimetric method
B.
UV kinetic method
Experiment no. 40 Determination of SGPT(ALAT) activity in serum
A.
Colorimetric method
B.
UV kinetic method
Experiment
no. 41 Determination of cholesterol in serum
A.
Enzymatic method (Kit Method)
B.
Chemical method (ZAK’s method)
Experiment no. 42 Quantitative determination of high-density lipoprotein cholesterol
Experiment no. 43 Quantitative determinations of triglycerides in serum
Experiment no. 44 Quantitative determination of low-density lipoprotein cholesterol
Enzymology
Experiment no. 45 Enzymatic hydrolysis of starch by amylase enzyme
A.
Effect of pH
B.
Effect of Temperature
C.
Effect of substrate concentration
D.
Effect of Time
Experiment
no. 46 Enzyme kinetics of protease enzyme
A.
Enzyme activity
B.
Effect of pH
C.
Effect of Temperature
D.
Effect of substrate concentration
E.
Effect of
Time
Annex I
Annex II
Annex III
Annex IV
Annex V
Annex VI
Annex VII
Annex VIII
Annex IX
Annex X
Bibiography
