Contents of Biochemistry Manual

Preface

Forward

Acknowledgements

List of Abbreviations

List of Tables

Lists of Figures

Format

Orientation on Instrumentation

Basic Techniques

Experiment no. 1              Preparation of different types of solutions (Percent, Molar, Normal, Solutions from Solutions and Titrations, Concentrated Solutions Saturated) used in biochemistry lab

Experiment no. 2              Preparation of different types of   Buffers used in biochemical Studies

Experiment no. 3              Determination of l_max of different colored and noncolored compounds by spectrophotometer

Experiment no. 4    Verification of validity of Beer’s law and determination of the molar extinction coefficient of BSA

Qualitative Methods

Experiment no. 5          Qualitative detection of proteins and amino acids

Experiment no. 6              Qualitative detection of carbohydrates

Experiment no. 7              Identification of the nature of

                                              carbohydrate in a given sample

Isolation and Quantitative Methods

Experiment no. 8              Titration curve for the given amino acids

Experiment no. 9           

A.       Quantitative determination of free  amino acid content in serum

B.       Comparative study of free amino acid content in germinating and non-germinating seeds

Experiment no. 10        Determination of Tyrosine content in a given solution using standard calibration curve

Experiment no. 11            Quantitative determination of protein

A.       Biuret method

B.       Bradford method

C.       Folin-Lowry method

D.       UV method

Experiment  no. 12           Isolation of Casein from milk

Experiment no. 13            Isolation and assay of glycogen from the liver and skeletal muscle

Experiment no. 14            Benedict’s test for reducing sugars

Experiment no. 15            Determination of soluble carbohydrates by Anthrone Method

Experiment no: 16            Determination of acid value of the given fats and oils

Experiment no: 17            Determination of the Iodine Number of lipids

Experiment no. 18           Quantitative analysis of DNA by flourimetry

Purification and Identification Methods

Experiment no. 19            Preparation of various sub-cellular fractions of liver cells by Differential Centrifugation method

Experiment no. 20            Fractionation of serum protein by salt (Sodium chloride and Ammonium sulphate to separate of albumin and globulin)

Experiment no. 21            Precipitation and separation of protein of interest by TCA (Trichloroacetic acid) method from biological sample

Experiment no. 22            Separation and identification of amino acids in a given mixture by ascending paper chromatography

Experiment no. 23            Separation and identification of amino acids in a given mixture by two dimensional paper chromatography

Experiment no. 24            Separation and identification of sugars (sugar juices) by adsorption Thin layer chromatography (TLC)

Experiment no. 25            Extraction and identification of lipids from given biological samples by Thin layer chromatography

Experiment no. 26            Purification of proteins by size exclusion chromatography (gel filtration)

Experiment no. 27            Separation of serum proteins by Ion-exchange Chromatography using cation exchanger

Experiment no. 28            Analysis of serum albumin by affinity chromatography using Affi-Gel Blue 

Experiment no. 29            Analysis of serum protein by electrophoresis using cellulose acetate paper

Experiment no. 30            Determination of molecular weight of a given protein by SDS-PAGE (Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis)

Experiment no. 31            Native PAGE (Poly Acrylamide Gel Electrophoresis) of proteins

Experiment no. 32            Determination of molecular weight of DNA (genomic and plasmid) by Agarose gel electrophoresis

Experiment no. 33            Detection of proteins and DNA in gels by Silver Staining

Clinical Biochemistry

Experiment no. 34            Determination of blood glucose

Experiment no. 35            Quantitative determination of total protein in serum and plasma

Experiment no. 36            Determination of Albumin in serum or plasma

Experiment no. 37            Determination of Direct and Total Bilirubin in serum

Experiment no. 38            Determination of hemoglobin by cyanmethemoglobin method

Experiment no. 39            Determination of SGOT (ASAT) activity in serum

A.       Colorimetric method

B.       UV kinetic method

Experiment no. 40            Determination of SGPT(ALAT) activity in serum

A.       Colorimetric method

B.       UV kinetic method

Experiment no. 41            Determination of cholesterol in serum

A.       Enzymatic method (Kit Method)

B.       Chemical method (ZAK’s method)

Experiment no. 42            Quantitative determination of high-density lipoprotein cholesterol

Experiment no. 43            Quantitative determinations of triglycerides in serum

Experiment no. 44            Quantitative determination of low-density lipoprotein cholesterol

Enzymology

Experiment no. 45            Enzymatic hydrolysis of starch by amylase enzyme

A.    Effect of pH

B.    Effect of Temperature

C.    Effect of substrate concentration

D.    Effect of Time

Experiment no.  46           Enzyme kinetics of protease enzyme

A.    Enzyme activity

B.    Effect of pH

C.    Effect of Temperature

D.    Effect of substrate concentration

E.    Effect of Time

Annex I

Annex II

Annex III

Annex IV

Annex V

Annex VI

Annex VII

Annex VIII

Annex IX

Annex X

Bibiography