DNA-RNA MCQ
1. A
peculiar cytochrome is observed in bacteria and it can react with molecular oxygen,
what is it?
a. Cyt b b.
Cyt c
c. Cyt d d.
Cyt o
2. The
genetic material in HIV is
a. ds DNA b.
ss DNA
c. s RNA d. None
of these
3. Which one
of the following mutagens act only on replicating DNA?
a. Ethidium bromide b.
Nitrosogeranidine
c. Acridine orange d.
None of above
4. Poly A
tail is frequently found in
a. Histone in RNA b.
Bacterial RNA
c. eukaryotic RNA d.
TRNA
5. Which of
the following is an example of RNA virus?
a. SV 40 b.
T4 phage
c. Tobacco mosaic virus d.
Adeno virus
6. Genomic
DNA is extracted, broken into fragments of reasonable size by a restriction
endonuclease and then inserted into a cloning vector to generate chimeric vectors.
The cloned fragments are called
a. Clones b.
Genomic library
c. mRNA d.
None of these
7.
Transgenic animals are produced when GH gene fused with
a. MT gene b.
GH
c. GRF d.
FIX
8. In which
medium the hydridoma cells grow selectively?
a. Polyethylene glycol
b. Hypoxanthine aminopterin thyminine
c. Hypoxathing-guaning phosphoribosyl
transferase
d. Both b and c
9. The
enzymes which are commonly used
in genetic
engineering are
a. Exonuclease and ligase
b. Restriction endonuclease and polymerase
c. Ligase and polymerase
d. Restriction endonuclease and ligase
10. A
successful hybridoma was produced by
fusing
a. Plasma cells and plasmids
b. Plasma cells and myeloma cells
c. Myeloma cells and plasmids
d. Plasma cells and bacterial cells
11. The technique
involved in comparing the DNA components of two samples is known as
a. Monoclonal antibody techniques
b. Genetic finger printing
c. Recombinant DNA technology
d. Polymerase chain reaction
12. Plasmids
are ideal vectors for gene cloning as
a. They can be multiplied by culturing
b. They can be multiplied in the laboratory using
enzymes
c. They can replicate freely outside the bacterial
cell
d. They are self replicating within the bacterial
cell
13. Humans
normally have 46 chromosomes in skin cells. How many autosomes would be
expected in a kidney cell?
a. 46 b. 23
c. 47 d. 44
14. Pasteur
effect is due to
a. Change from aerobic to anaerobic
b. Providing oxygen to anaerobically respiring
structures
c. Rapid utilization of ATP
d. Nonsynthesis of ATP
15. A
mechanism that can cause a gene to move from one linkage group to another is
a.Trans location b.
Inversion
c. Crossing over d.
Duplication
16. The
smallest unit of genetic material that can undergo mutation is called
a. Gene b.
Cistron
c. Replicon d.
Muton
17. The two
chromatids of metaphase chrosome represent
a. Replicated chromosomes to be separated at
anaphase
b. Homologous chromosomes of a diploid set
c. Non-homologous chromosomes joined at the
centromere
d. Maternal and paternal chromosomes joined
at the centromere
18. Malate
dehydrogenase enzyme is a
a. Transferase b.
Hydrolase
c. Isomerase d.
Oxido reductase
19. In E.
coli att site is in between
a. Gal and biogenes b.
Bio and niacin genes
c. Gal and B genes d.
None of these
20. The best
vector for gene cloning
a. Relaxed control plasmid
b. Stringent control plasmid
c. Both a and b
d. None of these
21. A gene
that takes part in the synthesis of polypeptide is
a. Structural gene b.
Regulator gene
c. Operator gene d.
Promoter gene
22. DNA
replicates during
a. G1 – phase b.
S – phase
c. G2 – phase d.
M – phase
23. A human
cell containing 22 autosome and a ‘Y’ chromosome is probably a
a. Male somatic cell b.
Zygote
c. Female somatic cell d.
Sperm cell
24. Crossing-over
most commonly occurs during
a. Prophase I b.
Prophase II
c. Anaphase I d.
Telophase II
25.
DNA-replication is by the mechanism of
a. Conservative b.
Semiconservative
c. Dispersive d.
None of the above
26.
Production of RNA from DNA is called
a. Translation b.
RNA splicing
c. Transcription d.
Transposition
27. Nucleic
acids contain
a. Alanine b.
Adenine
c. Lysine d.
Arginine
28. What are
the structural units of nucleic acids?
a. N-bases b.
Nucleosides
c. Nucleotides d.
Histones
29. The most
important function of a gene is to synthesize
a. Enzymes b.
Hormones
c. RNA d.
DNA
30. One of
the genes present exclusively on the X-chromosome in humans is concerned with
a. Baldness
b. Red-green colour baldness
c. Facial hair/moustache in males
d. Night blindness
31. Peptide
linkages are formed in between
a. Nucleotides b.
Amino acids
c. Glucose molecules d.
Sucrose
32. The
nucleic acid of polio viruses is
a. DNA b.
RNA – (+) type
c. t-RNA d.
m-RNA
33. Rabies
virus is
a. Nake RNA virus
b. Naked DNA virus
c. Enveloped RNA virus
d. Enveloped DNA virus
34. Example
for DNA virus:
a. Polio virus b.
Adeno virus
c. Echo virus d.
Poty virus
35. In
genetic engineering breaks in DNA are formed by enzymes known as
a. Restriction enzymes b.
Ligases
c. Nucleases d.
Hydralases
36. DNA
transfer from one bacterium to another through phages is termed as
a. Transduction b.
Induction
c. Transfection d.
Infection
37.
Microorganisms usually make acetyl CO-A by oxidizing
a. Acetic acid b.
Pyruvic acid
c. α-ketoglutaric acid d.
Fumaric acid
38. The
method of DNA replication proposed by Watson and Crick is
a. Semi conservative
b. Conservative
c. Dispersive
d. Rolling loop
39. The
distance between each turn in the helical strand of DNA is
a. 20 Ao b.
34 Ao
c. 28 Ao d. 42 Ao
40.
Self-replicating, small circular DNA molecules present in bacterial cell are known
a. Plasmids b.
Cosmids
c. Plasmomeros d.
plastides
41. Western
blotting is the technique used in the determination of
a. RNA b.
DNA
c. Proteins d.
All of these
42. m RNA
synthesis from DNA is termed
a. Transcription b.
Transformation
c. Translation d.
Replication
43. Western
blotting is a technique used in the determination of
a. DNA b.
RNA
c. Protein d.
Polysaccharides
44. Building
blocks of Nucleic acids are
a. Amino acids b.
Nucleosides
c. Nucleotides d.
Nucleo proteins
45. DNA
finger printing is based on
a. Repetitive sequences
b. Unique sequences
c. Amplified sequences
d. Non-coding sequences
46. The
enzyme required for DNA from RNA template:
a. RNA polymerase
b. Reverse transcriptase
c. DNA polymerase
d. Terminal transferase
47. Double
standard RNA is seen in
a. Reo virus b.
Rhabdo virus
c. Parvo virus d.
Retro virus
48. Example
for DNA viruses:
a. Adeno virus
b. Bacteriophage T1, T2, T3, T4, T5, T6
c. Papova virus
d. Herpes virus and cauliflower moisaic
e. All of the above
49. The
following are the RNA viruses, except
a. Reo viruses
b. Retro viruses
c. Bacteriophage Φ C
d. Tmv and Bacteriophages Ms2, F2
e. Dahila mosaic virus and Bacteriophages Φ
× 174, M12, M13
50. The two
strands of DNA are joined non covalently by
a. Ionic bonds
b. Covalent bonds
c. Hydrogen bonds between bases
d. Polar charges
51. The
bases Adenine and Thymine are paired with
a. Double hydrogen bonds
b. Single hydrogen bonds
c. Triple hydrogen bonds
d. Both b and c
52. The no.
of hydrogen bonds existing between Guanine and Cytosine are
a. 5 b.
2
c. 3 d.
None of these
53. The
length of each coil in DNA strand is
a. 15 Ao b.
34 Ao
c. 30 Ao d.
5 Ao
54. Nucleic
acids are highly charged polymers due to
a. There is phosphodiester bond between 5’-
hydroxyl of one ribose and 3’–hydroxyl of next ribose
b. They have positive and negative ends
c. Nucleotides are charged structures
d. Nitrogenous bases are highly ionized compounds
55. The best
studied example for specialized transduction is
a. P1 phage b.
P22 phage
c. ë-phage d.
Both a and c
56. The
diagrammatic representation of the total no. of genes in DNA is
a. Genome b.
Gene map
c. Gene-structure d.
Chromatin
57. During
specialized transduction
a. Large amound of DNA is transferred
b. A few no. of genes are transferred
c. Whole DNA is transferred
d. None of these
58. The cell
donating DNA during transformation is
a. Endogenate b.
Exogenate
c. Mesozygote d.
Merosite
59. Genetic
information transfer DNA to RNA is called –
a. Transcriptase b.
Transduction
c. Transformation d.
Recombination
60. The gene
transfer occurs by –
a. Transformation b.
Transduction
c. Conjugation d.
Cell fusion
(Citation from: Sagar G.V. (2008) MCQs in Microbiology, New Age International (P) Limited Publishers )
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