Monday, August 29, 2016

One-Day Symposium on "Microbiology and One Health Concept in Nepal"

One-Day Symposium
On
“Microbiology and One Health Concept in Nepal”

POSTER PRESENTATION-2016

Organized by Nepalese Society for Microbiology (NESOM)



POSTER FORMAT:

SIZE OF POSTER: 36″ x 48″ (3 feet x 4 feet) in Landscape style

MATERIAL OF POSTER: Flex printing

COLOR PRINTING

CONTENTS
·         As given below under the following heading; Heading, Abstract, Introduction, Materials and Methods, Results, Conclusion, References, Acknowledgements

HEADING: The heading should include; Title, Authors and Affiliations

Title

The title should be in bold, sentence case with no full stop at the end.
E.g.:  Intestinal Parasites among School Children in Bhaktapur District, Nepal
Authors                                  
Provide the name by which each contributor is known (First name, Middle name and Last name), with her/his institutional affiliation. Indicate the corresponding author with the name, address, mobile numbers and e-mail address. Where authors are from a number of different institutions, the appropriate institution number from the affiliation list should be given as a superscript number immediately after each author’s name. e.g.: Hari Shrestha1Sarita Lamichhane2, Namita Gurung3.

Affiliations      
Affiliations should includename of the institute, location anddistrict. Where there are multiple affiliations, each should be listed as a separate paragraph. Each institute should appear in the order used against the author names as mentioned above and show the appropriate superscript number.e.g.
1Kantipur College of Medical Science, Sitapaila, Kathmandu
2TrichandraMultiple Collage, Ghantaghar, Kathmandu
3 Central Department of Microbiology, Kirtipur, Kathmandu


Structured Posters:
ABSTRACT:
·         Words limit upto 300 words
·         Abstracts should be structured into following sections using the following headings typed in bold with no colon at the end, e.g.
·         Background with objectives, Methods, Results, Conclusion and Key words (3-5 words)
·         Should be at the top of the poster
·         NOTE: Abstract should be submitted separately for publication as well (within an official Hour-September 1, 2016 Thursday).

INTRODUCTION:
·         Brief introduction about the research
·         Should clearly mention the background, problem statement, rationale and justification of the study.

OBJECTIVES
·         Point wise
·         General and Specific

MATERIALS AND METHODS:
·         Brief methods and flowchart

RESULTS
·         Use of tables, bar graphs, pie chart for attractive presentation

CONCLUSION

·         Research conclusion in brief

REFERENCES 
·         Follow the guidelines for American Society for Microbiology (ASM) or TU thesis guidelines

ACKNOWLEDGEMENTS (OPTIONAL)
·         Only the list of name of persons who helped in the research

PROGRAM DETAILS:
Date:                                  September 3, 2016 (2073/05/18), Saturday
Venue:                               Hotel Orchid, Tripureshwor
Time:                                 8:00 AM (Registration)
                                           9:00 AM (Inauguration)
Registration fee:              Rs. 500/-

For Format of poster please visit the official website of NESOM or contact me on upedrats@gmail.com


Tuesday, August 9, 2016

A Practical Manual for Biochemistry and Instrumentation, Second Edition

Second Edition of Biochemistry and Instrumentation Practical Manual

CONTENTS
Title page
Publisher’s page
Preface
Foreword
Contents
List of Abbreviations
List of Tables
List of Figures
Practical format
Orientation on instruments
i
ii
iii
v
vi
x
xiii
xv
1
3


Experiment no. 1




Experiment no. 2

Experiment no. 3


Experiment no. 4   




Experiment no. 5   

Experiment no. 6            
Experiment no. 7    



Experiment no. 8   
Experiment no. 9(A)

Experiment no. 9(B)

Experiment no. 10  
  
Experiment no. 11 




Experiment  no. 12
Experiment no. 13

Experiment no. 14
Experiment no. 15

Experiment no. 16

Experiment no. 17
Experiment no. 18

Experiment no. 19

         
Experiment no. 20  

     
Experiment no. 21


Experiment no. 22


Experiment no. 23


Experiment no. 24


Experiment no. 25

               
Experiment no. 26      


Experiment no. 27

Experiment no. 28


Experiment no. 29

Experiment no. 30
               
Experiment no. 31



Experiment no. 32

Experiment no. 33


Experiment no. 34



Experiment no. 35
Experiment no. 36

Experiment no. 37
Experiment no. 38

Experiment no. 39

Experiment no. 40



Experiment no. 41



Experiment no. 42
               


Experiment no. 43

Experiment no. 44

Experiment no. 45



Experiment no. 46
               


Experiment no.  47




Annex I
Annex II
Annex III
Annex IV
Annex V
Annex VI
Annex VII
Annex VIII
Annex IX
Annex X
Annex XI
Annex XII

Annex XIII
Annex XIV
Bibliography

Basic Techniques
Preparation of different types of solutions (Percent, Molar, Normal, Solutions from Solutions and Titrations, Concentrated Solutions Saturated) used in biochemistry laboratory
Preparation of different types of   Buffers used in biochemical Studies
Determination of lmax of different colored and noncolored compounds by spectrophotometer
Verification of validity of Beer’s law and determination of the molar extinction coefficient of BSA

Qualitative Methods
Qualitative detection of proteins and amino acids
Qualitative detection of carbohydrates
Identification of the nature of
 carbohydrate in a given sample

Isolation and Quantitative Methods
Titration curve for the given amino acids
Quantitative determination of free  amino acid content in serum
Comparative study of free amino acid content in germinating and non-germinating seeds
Determination of Tyrosine content in a given solution using standard calibration curve
Quantitative determination of protein
A.       Biuret method
B.       Bradford method
C.       Folin-Lowry method
D.      UV method
Isolation of Casein from milk
Isolation and assay of glycogen from the liver and skeletal muscle
Benedict’s test for reducing sugars
Determination of soluble carbohydrates by Anthrone Method
Determination of acid value of the given fats and oils
Determination of the Iodine Number of lipids
Determination of saponification value of fats and oils
Quantitative analysis of DNA by flourimetry

Purification and Identification Methods
Preparation of various sub-cellular fractions of liver cells by Differential Centrifugation method
Fractionation of serum protein by salting out using Sodium chloride and Ammonium sulphate
Precipitation and separation of protein of interest by Trichloroacetic acid method from biological sample
Separation and identification of amino acids in a given mixture by Ascending Paper Chromatography
Separation and identification of amino acids in a given mixture by Two-dimensional Paper Chromatography
Separation and identification of sugars (sugar juices) by adsorption Thin layer chromatography (TLC)
Extraction and identification of lipids from given biological samples by Thin Layer Chromatography
Purification of proteins by Size Exclusion Chromatography
Separation of serum proteins by Ion-exchange Chromatography using cation exchanger
Purification of proteins by Affinity Chromatography
Analysis of serum protein by Paper Electrophoresis using cellulose acetate paper
Determination of molecular weight of a given protein by Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Native PAGE (Poly Acrylamide Gel Electrophoresis) of proteins
Determination of molecular weight of DNA (genomic and plasmid) by Agarose gel electrophoresis
Detection of proteins and DNA in gels by Silver Staining

Clinical Biochemistry
Determination of blood glucose
Quantitative determination of total protein in serum and plasma
Determination of Albumin in serum or plasma
Determination of Direct and Total Bilirubin in serum
Determination of hemoglobin by cyanmethemoglobin method
Determination of Serum Glutamic Oxalocetic Transminase (SGOT) activity in serum
A.       Colorimetric method
B.       UV kinetic method
Determination of Serum Glutamic Pyruvic Transaminase (SGPT) activity in serum
A.       Colorimetric method
B.       UV kinetic method
Determination of cholesterol in serum
A.       Enzymatic method (Kit Method)
B.       Chemical method (ZAK’s method)

Quantitative determination of high-density lipoprotein cholesterol
Quantitative determinations of triglycerides in serum
Quantitative determination of low-density lipoprotein cholesterol

Enzymology
Enzyme activity of protease and study the effects of temperature, pH, enzyme concentration, time and effectors on the kinetics of protease catalyzed reactions
Study of effect of substrate concentration and determination of Vmax and Km value of a-amylase

Annexes
Buffer
Spectroscopy
Carbohydrates
pKa value
Milk
Lipids
Centrifugation
Salting out
Chromatography media
Buffer systems for chromatography
Polyacrylamide gel electrophoresis
Molecular weight determination of protein by SDS-PAGE
DNA electrophoresis
pH indicators






5

9


12


14



17
19

26


28

31

33

35
38
38
39
41
42
44

47
49

52

54
56

58
60




63


65


68


70


73


76


79

82


85

87

90



92

97


99

101


107

109
111

113

116


119
121


124
126

129
131


133

136

139





142


151


156
160
161
162
163
164
166
168
170
173175

178
179
181
182

A Practical Manual for Microbial Genetics

New Book on Practical Microbial Genetics


TABLE OF CONTENTS
Contents

Orientation of Microbial genetics / Molecular Biology Laboratory

Theory
Micropipettes
DNA Extraction
Role of Sodium in DNA Extraction
Restriction Enzymes
Plasmid
Bacteriophages
Polymerase Chain Reaction-PCR








Experiment no. 1
Calibration of Micropipettes

Experiment no. 2
Preparation of different Tris buffers: Tris-HCl, Trsi-EDTA and TAE

Experiment no. 3
Isolation of DNA from human blood

Experiment no. 4a
Extraction of chromosomal DNA from Gram positive bacteria

Experiment no. 4b
Extraction of chromosomal DNA from Gram negative bacteria

Experiment no. 5a
Plasmid DNA extraction from bacteria (Alkaline lysis method)

Experiment no. 5b
Isolation and purification of bacterial plasmid with Qiagen spin (QIAprep) Miniprep system

Experiment no. 6
RNA extraction from yeast

Experiment no. 7
Spectrophotometric analysis of DNA in extracted solution

Experiment no. 8
Agarose gel electrophoresis of DNA

Experiment no. 9a
Demonstration of induction / repression mode of lac-operon regulation system in Escherichia coli
     
Experiment no. 9b
Determination of level of induction of lac-operon in Escherichia coli

Experiment no. 10
Conjugation in bacteria: Co-culture of MDR Salmonella enteritidisTyphi with Escherichia coli HB 101 and detection of transconjugants by antibiotic selection method

Experiment no. 11
Transformation of Escherichia coli HB101 by plasmids: pUC18 or pBR322

Experiment no. 12
Study of performance of HindIII and EcoRI restriction enzymes

Experiment no. 13a
Construction of recombinant DNA: Preparation and purification of insert (Lambda DNA fragments) and vector (pUC18) DNA

Experiment no.13b
Construction of recombinant DNA: Ligation of purified insert (Lambda DNA fragments) and vector (pUC18) DNA

Experiment no. 14a
Amplification of recombinant DNA: Transformation of recombinant pUC18 DNA in Escherichia coli HB101

Experiment no.14b
Detection of recombinant DNA: Extraction of recombinant pUC18 DNA from transformed Escherichia coli HB101 clones

Experiment no. 14c
Detection of recombinant DNA: Extraction of recombinant pUC18 DNA in plasmid extracts of transformed Escherichia coli HB101 clones by agarose gel electrophoresis

Experiment no. 15a
Amplification of Escherichia coli DNA (genes) by Polymerase chain Reaction (PCR)

Experiment no.15b
Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)


Bacteria in Photos

Bacteria in Photos