Second Edition of Biochemistry and Instrumentation Practical Manual
CONTENTS
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Title page
Publisher’s
page
Preface
Foreword
Contents
List of
Abbreviations
List of
Tables
List of
Figures
Practical
format
Orientation
on instruments
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i
ii
iii
v
vi
x
xiii
xv
1
3
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Experiment
no. 1
Experiment
no. 2
Experiment
no. 3
Experiment
no. 4
Experiment
no. 5
Experiment
no. 6
Experiment
no. 7
Experiment
no. 8
Experiment no. 9(A)
Experiment no. 9(B)
Experiment
no. 10
Experiment
no. 11
Experiment no. 12
Experiment
no. 13
Experiment
no. 14
Experiment
no. 15
Experiment
no. 16
Experiment
no. 17
Experiment
no. 18
Experiment no. 19
Experiment
no. 20
Experiment
no. 21
Experiment
no. 22
Experiment
no. 23
Experiment
no. 24
Experiment
no. 25
Experiment
no. 26
Experiment
no. 27
Experiment
no. 28
Experiment
no. 29
Experiment
no. 30
Experiment
no. 31
Experiment
no. 32
Experiment
no. 33
Experiment
no. 34
Experiment
no. 35
Experiment
no. 36
Experiment
no. 37
Experiment
no. 38
Experiment
no. 39
Experiment
no. 40
Experiment
no. 41
Experiment
no. 42
Experiment
no. 43
Experiment
no. 44
Experiment
no. 45
Experiment
no. 46
Experiment
no. 47
Annex I
Annex II
Annex III
Annex IV
Annex V
Annex VI
Annex VII
Annex VIII
Annex IX
Annex X
Annex XI
Annex XII
Annex XIII
Annex XIV
Bibliography
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Basic Techniques
Preparation of different types of
solutions (Percent, Molar, Normal, Solutions from
Solutions and Titrations, Concentrated
Solutions Saturated) used in biochemistry laboratory
Preparation of different types
of Buffers used in biochemical
Studies
Determination of lmax of different colored and noncolored compounds by
spectrophotometer
Verification of validity of Beer’s
law and determination of the molar extinction coefficient of BSA
Qualitative Methods
Qualitative detection of proteins
and amino acids
Qualitative detection of
carbohydrates
Identification of the nature of
carbohydrate in a given sample
Isolation and Quantitative Methods
Titration curve for the given amino
acids
Quantitative determination of
free amino acid content in serum
Comparative study of free amino acid
content in germinating and non-germinating seeds
Determination of Tyrosine content in
a given solution using standard calibration curve
Quantitative determination of
protein
A.
Biuret method
B.
Bradford method
C.
Folin-Lowry method
D.
UV method
Isolation of Casein from milk
Isolation and assay of glycogen from
the liver and skeletal muscle
Benedict’s test for reducing sugars
Determination of soluble
carbohydrates by Anthrone Method
Determination of acid value of the
given fats and oils
Determination of the Iodine Number
of lipids
Determination
of saponification value of fats and oils
Quantitative
analysis of DNA by flourimetry
Purification and Identification Methods
Preparation of various sub-cellular
fractions of liver cells by Differential Centrifugation method
Fractionation of serum protein by
salting out using Sodium chloride and Ammonium sulphate
Precipitation and separation of
protein of interest by Trichloroacetic acid method from biological sample
Separation and identification of
amino acids in a given mixture by Ascending Paper Chromatography
Separation and identification of
amino acids in a given mixture by Two-dimensional Paper Chromatography
Separation and identification of
sugars (sugar juices) by adsorption Thin layer chromatography (TLC)
Extraction and identification of
lipids from given biological samples by
Thin Layer Chromatography
Purification of proteins by Size Exclusion
Chromatography
Separation of serum proteins by
Ion-exchange Chromatography using cation exchanger
Purification of proteins by Affinity
Chromatography
Analysis of serum protein by Paper Electrophoresis
using cellulose acetate paper
Determination of molecular weight of
a given protein by Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis
(SDS-PAGE)
Native PAGE (Poly Acrylamide Gel
Electrophoresis) of proteins
Determination of molecular weight of
DNA (genomic and plasmid) by Agarose gel electrophoresis
Detection of proteins and DNA in
gels by Silver Staining
Clinical Biochemistry
Determination of blood glucose
Quantitative determination of total
protein in serum and plasma
Determination of Albumin in serum or
plasma
Determination of Direct and Total
Bilirubin in serum
Determination of hemoglobin by
cyanmethemoglobin method
Determination of Serum Glutamic
Oxalocetic Transminase (SGOT) activity in serum
A.
Colorimetric method
B.
UV kinetic method
Determination of Serum Glutamic
Pyruvic Transaminase (SGPT) activity in serum
A.
Colorimetric method
B.
UV kinetic method
Determination of cholesterol in
serum
A.
Enzymatic method (Kit Method)
B.
Chemical method (ZAK’s method)
Quantitative determination of
high-density lipoprotein cholesterol
Quantitative determinations of
triglycerides in serum
Quantitative determination of
low-density lipoprotein cholesterol
Enzymology
Enzyme activity of protease and
study the effects of temperature, pH, enzyme concentration, time and
effectors on the kinetics of protease catalyzed reactions
Study of effect of substrate
concentration and determination of Vmax and Km value of
a-amylase
Annexes
Buffer
Spectroscopy
Carbohydrates
pKa value
Milk
Lipids
Centrifugation
Salting out
Chromatography media
Buffer systems for chromatography
Polyacrylamide gel electrophoresis
Molecular weight determination of
protein by SDS-PAGE
DNA electrophoresis
pH indicators
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