A Complete Note on Instrumentation (Analytical Biochemistry)
UPENDRA THAPA SHRESTHA
A Complete Note
on Instrumentation (Analytical Biochemistry)
First Edition,
November-2016
© Author (Shrestha U.T.)
UPENDRA THAPA SHRESTHA, M.Sc.
Microbiology (TU)
Head of Microbiology
Department: Kantipur College of Medical Science, Sitapaila, KTM
(B.Sc. and M.Sc. Microbiology affiliated to TU;
CMLT and D-Pharmacy affiliated to CTEVT)
Deputy Director: Research Laboratory for Biotechnology and
Biochemistry (RLABB)
(Research Scientist)
Faculty: Khwopa College (B.Sc. and M.Sc. Environment
Science), Dekocha, Bhaktapur
PGT: Sainik Awasiya Mahavidhyalaya, Sallaghari, Bhaktapur
Email: upendrats@gmail.com
Publisher:
All
rights reserved.
No part of this book may be reproduced
in any form, by photocopy, microfilm, scanning or any other means, or
incorporated into any information retrieval system, electronic or mechanical,
without the written permission of the author.
Thank you!
UNIT-I
·
1.1 Physiological
Solutions
·
1.2
Cell Disruption
o
Mechanical
Cell Disruption
o
Non-mechanical
Cell Disruption
§ Physical Methods
§ Chemical Methods
§ Biological Methods
UNIT-II
·
Protein
Purification
o
Background
knowledge about protein
o
Protein
Isolation/Precipitation
o
Protein
Purification
o
Protein
Determination
o
Protein
Assay
UNIT-III
·
Centrifugation
o
Principle
of Centrifugation
o
Centrifuges
and its types
o
Differential
Centrifugation
o
Density
Gradient Centrifugation
UNIT-IV
·
Chromatography
o
Paper
Chromatography
o
Thin
Layer Chromatography
o
Column
Chromatography
§ Size Exclusion Chromatography
§ Ion Exchange Chromatography
§ Affinity Chromatography
§ Gas Chromatography
§ High Performance Liquid Chromatography (HPLC)
UNIT-V
·
Electrophoresis
o
Principle
of Electrophoresis
o
Media
used in Electrophoresis
o
Agarose
Gel Electrophoresis
o
Isoelectric
Focus Gel Electrophoresis
o
SDS-PAGE
o
2D
Gel Electrophoresis
o
Western
Blotting
UNIT-VI
·
Spectroscopy
o
UV-Visible
Spectrophotometry
§ Colorimeter/Photometer/Spectrophotometer
o
Spectroflouremetry
o
Molecular
Luminescence Spectroscopy
o
Atomic
Spectroscopy
§ Atomic Absorption Spectroscopy
§ Atomic Emission Spectroscopy
§ Atomic Florescent Spectroscopy
o
Mass
Spectroscopy
o
Nuclear
Magnetic Resonance
UNIT-VII
·
Microscopy
o
Electron
Microscopy
§ Transmission Electron Microscopy
§ Scanning Electron Microscopy
o
Phase
Contrast Microscopy
o
Fluorescent
Microscopy
o
Gel-Doc
System
UNIT-VIII
·
Polymerase
Chain Reaction
·
Microarrays
5 comments:
your information is really awesome as well as it is very excellent and i got more interesting information from your blog. Xitij instrument all different type of models rapid midi dual wet blot for different applications like Junior,Mini, Midi, Maxi & Customized Electrophoresis.They provide the best services in pune.
Rapid midi dual wet blot
western blot
Thanks for sharing!
At Xitij Instruments we provid state of art UV Transilluminator and other lab equipments.
But the note is not complete
This is really amazing blog...thanks for sharing
Microbiology Laboratory Equipment
A locked and restricted crypto account but that did not stop me from the recovery of my crypto assets. I was locked out of my wallet, and restricted for reasons they couldn’t explain to me properly. I thought I had lost everything but a friend of mine recommended MorphoHack to me. A team of cyber security experts, crypto recovery specialists and ethical hackers who helped my case. In my situation, I was about to swindled by the crypto holding company, but MorphoHack helped me recover my crypto assets, gained access to my wallet and helped me regained my confidence. I’m truly thankful for their service and I highly recommend them to everyone out there. Contact them directly at Morphohack@cyberservices.com
Many people are afraid to pursue it further once they lose access to their crypto wallet and assets, but I put it to you that the longer you wait, the more complicated it becomes. Morphohack can help you act faster and more efficiently, you will be glad that you stumbled upon their services.
Post a Comment