Friday, August 24, 2018

Article published in JHAS but missed to be posted


Wagle et. al. Antibiotic Sensitivity Pattern and Plasmid Profile Of Urinary Tract ....., JHAS, 2016, Vol. 5, No. 1 P 58-62

Antibiotic Sensitivity Pattern and Plasmid Profile of Urinary Tract Infection Isolates among Children Below 10 Years Of Age

Ram Wagle1, Rajendra Timilsina1, Rojesh Thapa1, Nabaraj Adhikari2, Upendra Thapa Shrestha2, Suresh Jaiswal1, Bishnu Raj Tiwari1,
1School of Health and Allied Sciences, Pokhara University, Kaski, Nepal
2Kantipur College of Medical Sciences, Sitapaila, Kathmandu, Nepal

ABSTRACT
Urinary tract infection (UTI) is defined as colonization of pathogen anywhere along the urinary tract. UTI has been classified by site of infection as Upper urinary tract infection and lower urinary tract infection and by severity as Complicated and uncomplicated UTI. This community based cross sectional study was conducted to determine the Antibiotic sensitivity pattern and plasmid profile of most prevalent urinary tract infection isolates among children below 10 years of age, from December 2013 to February 2014. Among the total 800 sample collected from the community 390(48.8%) were female and 410(51.2%) were male. The prevalence rate was found to be 44(5.5%) of total cases and was statistically significant (p value is less than 0.05). Among significant growth, 15(1.9%) and 29(3.6%) were male and female respectively (p value is less than 0.05). Out of 44 total isolates frequency of Escherichia coli (E. coli) was 20 followed by Staphylococcus aureus (12), which accounted for 45.5% and 27.3% respectively. Remaining were Pseudomonas (11.4%), Klebsiella (11.4%), Proteus (2.3%) and Citrobacter sps. (2.3%). Tobramycin (100%) and Amikacin (97.73%) were found to be the most sensitive antibiotics followed by Chloramphenicol (93.18%), Imipenem (90.91%) and Ciprofloxacin (75%) respectively. Out of 20 E. coli isolates, no plasmid was seen in 7(35%) while 8(40%) showed single plasmid which was present in 8 isolates. Plasmid copy number of 2, 3 and 4 were displayed by 2(10%), 2(10%) and 1(5%) of the isolates respectively. A common (greater than 21 kilobases) plasmid was the most common among isolates under study. This study revealed that E. coli was the most prevalent organism causing community acquired pediatric UTI. Antibiotics that are commonly used for the management of UTI and other cases are being more resistant i.e., Ampicillin. Plasmid analysis showed the presence of plasmids in resistant E. coli isolates that might harbor resistant genes. So that further analysis is required for the detection of responsible genes.


Key words: Antibiotic sensitivity pattern, Plasmid profile, Urinary tract infection 

NOTE: Full text is available at RESEARCH GATE.

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