Upendra Thapa
Shrestha # 1 2, Sabnum Shrestha # 1, Nabaraj Adhikari 1 2, Komal Raj Rijal 2, Basudha Shrestha 3, Bipin Adhikari 4, Megha Raj
Banjara 2, Prakash Ghimire 2
Affiliations expand
·
PMID: 32606839
PMCID: PMC7320882
·
DOI: 10.2147/IDR.S250591
Free
PMC article
Abstract
Introduction: Extended-spectrum β-lactamases (ESBL) among Gram-negative
bacteria, predominantly Escherichia coli (E. coli), in
Nepal, have been rising. The main objectives of this study were to determine
the prevalence of uropathogenic E. coli, antibiotic resistance,
ESBLs, ABLs (AmpC type β-lactamases), MBLs (metallo-β-lactamases) and KPCs (Klebsiella
pneumoniae carbapenemases) and their correlation with plasmid
profiling patterns among patients with urinary tract infections in a tertiary
hospital in Kathmandu, Nepal.
Methods: The mid-stream urine samples collected from patients were
inoculated in cystine-lactose-electrolyte-deficient (CLED) agar plates. E.
coli producing ESBLs, ABLs, MBLs/KPC were identified phenotypically
using standard microbiological methods. Plasmids were extracted by alkaline
lysis method from E. coli isolates and profiled using agarose
gel electrophoresis.
Results: Out of the total 2661 urine samples, E. coli were
isolated in 64.34% (507/788), among which 170 (33.53%) were multidrug-resistant
(MDR) isolates. All MDR isolates were resistant to amoxicillin and
third-generation cephalosporins but were highly sensitive to imipenem (94.12%,
160/170), amikacin (92.94%, 158/170) and nitrofurantoin (86.47%, 147/170).
Among 170 MDR isolates, 78.2% (133/170) were ESBLs, 46.3% (50/170) were AmpC,
11.2% (19/170) were MBL and 0.6% (1/170) were KPC producers. Coproduction of
β-lactamases was detected in 24.12% (41/170) of isolates. E. coli isolates
showed one plasmid (>33.5 kb), which was present in all the isolates.
Overall, 44 different plasmid profile groups were identified based on molecular
weight and number of plasmids. β-Lactamase producers were relatively resistant
to the higher number of antibiotics tested (≤10) than non-producers (≤8), and
the number of plasmids were higher in β-lactamase producers (≤7) than those in
non-producers (≤5).
Conclusion: The higher prevalence of the ESBLs, AmpCs, KPCs and MBLs along
with their coproduction in E. coli isolates highlights the
importance of routine surveillance of ESBLs, AmpCs, KPCs and MBLs in microbiology
laboratories using various phenotypic methods.
Keywords: AmpC type β-lactamases; ESBL; KPC; Klebsiella pneumoniae
carbapenemases; MBL; antibiotic resistance; extended-spectrum β-lactamases;
metallo-β-lactamases; uropathogenic Escherichia coli.
Fulltext Article: Download
24 comments:
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before buying anything online, try get your tools ready.. you can check on him on telegram weather he is still in business
Telegram- @darknetangel
icq chat- 746825067
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SPARK! CRYPTOCURRENCIES PRIVATE KEYS SOFTWARE - $500 Bitcoin
SPARK! CREDIT CARD HACK SOFTWARE - $500 Bitcoin
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www.vidlii.com/watch?v=2nJ44BSyS3P
www.vidlii.com/watch?v=lUu54scCYU9
blackeels@outlook.com
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Telegram= @leadsupplier @killhacks @jacobfullz
ICQ= 752822040 / @killhacks
Email= hacksp007 @ dnmx.org
FRESH FULLZ/PROS AVAILABLE
CC with CVV
SSN DOB DL
High Credit Scores Pros
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Dumps with PIN Codes Track 101 & 202
DL Scan Front & Back
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USA, UK, CANADA Fullz Available
Fresh & Legit stuff
No refund only replacement
This is for anyone seeking to recover their data or funds lost to scammers on the internet. I sent out $132,000.00 worth of BTC to this broker named E29 Crypto Marketers. My experience was a good business proposal turned sour. l was added to a crypto learning and trading group on Telegram. I thought it was real, unfortunately, they are like other fraudsters found on the internet and online romance apps, I only knew about their fraudulent activities after losing my investment to E-29 marketers. l would have lost all my invested funds to this online scam scheme. with the quick help of these professionals in reclaiming stolen money. I found their reviews on Google, After my research about them, I concluded they were the perfect recovery expert to hire to recover my stolen cryptos. I never regret coming in contact with CYBER GENIE HACK PRO team. Within the short period I spent conversing with them during the recovery process, I learned a lot more than the four walls of a school. They can be reached via their details below: Watsapp; +1 (252) 512-0391 Telegram; CYBERGENIEHACKPRO... Web; cybergeniehackpro (.) xyz/
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****Contact****
*ICQ :748957107
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USA SSN FULLZ WITH ALL PERSONAL DATA+DL NUMBER
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Young age data
Any age range data available
UK data-Canada data
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*ICQ :748957107
*Telegram :@James307
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