Antimicrobial Activity of Traditional Medicinal Plants Available at Banepa and Bhaktapur against Uropathogens
Susma
Dahal1#, Renuka Thapa1#, Anisha Suwal1#,
Dinesh Dhakal1, Alina Singh2, Milan Kumar Upreti3,
Upendra Thapa Shrestha4 *
1Sainik Awasiya Mahavidhyalaya
(affiliated to Tribhuvan University) Sallaghari, Bhaktapur, Nepal
2Department of Laboratory
Medicine, Shree Birendra Hospital, Chhauni, Kathmandu, Nepal
3Department of Microbiology,
GoldenGate International College, Battisputali, Kathmandu, Nepal
4Central Department of
Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
# All
authors have equally contributed in the research work.
*Corresponding author:
Upendra Thapa Shrestha, Central Department of Microbiology, Tribhuvan
University, Kirtipur, Kathmandu, Nepal, Email:
upendra.thapashrestha@cdmi.tu.edu.np
ABSTRACT
Objectives:
The study was aimed to determine the antimicrobial activity of traditional
medicinal plants against the uropathogens.
Methods: Overall,
360 urine samples were collected from both outpatient and inpatient for culture
and antimicrobial susceptibility testing. All the isolates were processed and
identified following standard microbiological procedure and subjected to
antibiotic susceptibility testing at Microbiology laboratory of Shree Birendra
Hospital following CLSI guidelines. All the three plant extracts were processed
by agar well diffusion method and Tube dilution method for antimicrobial
activity against Escherichia coli, Klebsiella. pneumoniae, Pseudomonas
aeruginosa and Enterobacter spp. at Microbiology laboratory of Sainik
Awasiya Mahavidhyalaya following standard laboratory techniques.
Results: Crude
extract of plants viz. Centella asiatica, Cuscuta reflexa and Mentha spicata
showed good antimicrobial properties against all clinical isolates. Among
all plants, ethanolic extract of C. asiatica was found to be most
effective against E. coli with zone of inhibition 16 mm and minimum
inhibitory concentration (MIC) value 5 mg/ml. Acetone extract of C. reflexa
showed good antimicrobial activity against K. pneumoniae with zone of
inhibition 14 mm and MIC value 10 mg/ml.
Conclusion:
Our research revealed that the crude plant extracts, particularly the acetone
and ethanol extracts, had a considerable amount of efficacy against uropathogens.
Based on the study results, these traditionally used medicinal plants can
overcome the problems of infections caused by multidrug resistant bacteria.
Keywords: Urinary tract infection, antimicrobial
activity, Medicinal plant, uropathogens, multidrug-resistant
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