Bikrant Gumanju1,
Roshna Shrestha1, Poonam Lakhemaru1, Rakysa Upadhyaya1,
Sushila Shrestha1, Dinesh Dhakal1, Upendra Thapa Shrestha2*
1Department of Microbiology, Sainik Awasiya Mahavidhyalaya, Sallaghari,
Bhaktapur
2Central Department of Microbiology, Tribhuvan University, Kirtipur,
Kathamandu
*Corresponding author: Upendra Thapa Shrestha, Assistant Professor,
Central Department of
Microbiology, Tribhuvan University, Kirtipur,
Kathmandu, E-mail: upendrats@gmail.com
ABSTRACT
Objectives: The present study aimed to identify bacteria profile of
cell phones used by different people from different profession of Bhaktapur
and to access their antimicrobial resistance.
Methods: Forty-nine mobile swab samples were collected from 7
different profession category (7 samples each from student, butcher, cook, panipuri
vendor, health workers, and dairy employee). Samples were cultured and
processed by standard Microbiological procedures. All the isolates were further
subjected to antibiotic susceptibility testing using modified Kirby Bauer disc
diffusion method as describe in CLSI guidelines. The rate of multiple drug
resistant (MDR) bacteria was also determined.
Results: Out of 49 sample, Bacillus spp (20.4%) was the most predominant isolate,
followed by
Staphylococcus aureus (10.6%) and Pseudomonas spp (10.6%). Higher variety of bacterial isolates was found in the cell
phones swabs from butcher followed by cook, farmer and panipuri vendor group. The
data from the questionnaire showed that handkerchief or tissue paper were
mostly used by respondents to clean their mobile. All Gram-positive and
Gram-negative isolates were resistance to Cefoxitin (100%) except Micrococcus spp and Neisseria spp. Gram positive (18.2%) and Gram-negative (36.9%) isolates were
identified as MDR. All S.
aureus and coagulase negative
staphylococci were methicillin resistant
Conclusion: The cell phones of people from different profession
were found to possess many different bacterial pathogens including multi drug
resistant strains which could be the possible pathogens for food borne
infections and opportunistic infections.
Key words: Cell phones, Antimicrobial susceptibility test, MDR,
Standard microbiological procedure
Date of
Submission: October 3, 2019
Date of Acceptance: November 27, 2019
Date of Acceptance: November 27, 2019
Citation: Gumanju
et al. 2019, Bacterial
Profile and Their Antibiogram Isolated from Cell Phones
TUJM 6(1): 96-102
